Fractional Equipment for Cell and Molecular Biology

9818124摘要本多用户方案是对细胞分子生物学研究和教学中组织分离和分析所需基本设备的需求。设备包括：1。分离和分离非常小的亚细胞结构、细胞蛋白、病毒、DNA等的一种超离心机。一种高速冷冻离心机和转子，用于准备分离和分离较大的细胞部分。具有温度控制样品室的紫外/可见分光光度计，用于酶研究、化学分析和DNA分析。一种均质机，用于快速处理组织样品，样品与样品之间的均匀性高。研究级水净化系统，用于生产高质量的水，用于敏感的化学测试、组织培养和DNA分析。三名教员将成为新设备的主要用户，以扩大他们当前研究的范围，使其能够进行目前无法进行的分析。能力的提高和研究的新途径也将与更好地培养本科生和研究生联系在一起。在微生物学课程以及植物、动物和细胞生理学课程中，可能会用到重要的实验室技术来分离亚细胞结构和分子。特别是，这些课程将能够包括分子生物学和生物技术中使用的许多现代技术。Sasek正在研究臭氧胁迫对植物的影响。臭氧是空气污染的一个重要组成部分，对人类健康和植物生产力构成重大威胁。未来的臭氧污染还可能与大气中二氧化碳浓度上升和全球变暖相互作用。新设备将有可能表征重要的植物防御机制和修复过程。一个主要目标是确定不同遗传系的敏感性范围。这将有助于更好地理解植物对多种胁迫的反应。芬德利正在研究微孢子虫，这是一种生活在各种动物细胞内的高度特化的单细胞寄生虫。其中许多是重要的病原体，但我们对它们的了解还不够透彻。新的设备将允许目前的研究扩大到包括更多的生理研究和代谢分析，以更好地表征生物体。特别是，将有可能在生命周期的几个不同发育阶段进行一系列研究，进一步有助于了解寄生对宿主细胞的影响。赫克特正在对有蹄动物（绵羊、山羊等）的逆转录病毒进行研究，以研究这些病毒的进化史。新设备将使充分探索表征某些基因序列所必需的大多数重要分子生物学技术成为可能。正在研究特定病毒序列的运动和传播，以确定哪些病毒是古老的，因此在许多品种和物种中都有发现，哪些是现代的，仍在积极传播。这种方法对于更好地了解其他重要病毒性疾病的传播也很有价值。NLU是服务于密西西比三角洲下游的主要机构，这是该国最贫困的经济地区之一。该大学最重要的贡献之一是通过帮助弥合文化和经济差距来改善当地人口和社区的状况。提供现代、最新的生物科学研究和教学机会的能力至关重要，不仅对生物专业，而且对许多药学和相关健康科学专业的学生都有积极的影响。基础设施的改善也影响了生物学中许多代表性不足的群体。作为一所开放招生的大学，NLU满足了多元化社区的需求，因此与选择性招生大学相比，NLU提供了更高比例的代表性不足群体的入学机会。

9818124abstract This multi-user proposal is a request for basic equipment needed for fractionating and analyzing tissues in cell and molecular biology research and teaching. The equipment includes:1. An ultracentrifuge for the fractionation and isolation of very small subcellular structures, cell proteins, viruses, DNA, etc.2. A highspeed refrigerated centrifuge and rotors for preparatory separations and isolation of larger cell parts.3. A UV/Vis spectrophotometer with temperature-controlled sample chamber for enzyme studies, chemical analyses, and DNA analysis.4. A homogenizer to process tissue samples quickly and with high sample-to-sample uniformity.5. A research-grade water purification system to produce high-quality water necessary for sensitive bichemical tests, tissue culture, and for DNA analyses.Three faculty members will be the major users of the new equipment to extend the range of their current research by making it possible to perform analyses currently not possible. Improved capabilities and newavenues of research will also be tied to better training of undergraduate and graduate students. In microbiology courses and in plant, animal, and cell physiology courses, important laboratorytechniques to isolate and separate subcellular structures and molecules will be possible. In particular, these courses will be able to include many of the modern techniques used in molecular biology andbiotechnology. Sasek is studying the effects of ozone stress on plants. Ozone, an important component of air pollution, is a significant threat to human health as well as plant productivity. Ozone pollution in the future mayalso interact with rising CO2 concentrations in the atmosphere and global warming. The new equipment would make it possible to characterize important plant defense mechanisms and repair processes. One major goal is to determine the range of sensitivity in different genetic lines. This will contribute to a better understanding of plant responses to multiple stresses.Findley is studying the microsporidia, a large group of highly-specialized single-celled parasites that live inside the cells of various animals. Many are important pathogens that are not well understood. The new equipment will allow current research to be expanded to include more physiological studies and metabolic analyses to characterize the organisms better. In particular, it will be possible to conduct a range of studies at several different developmental stages in the life cycle, further contributing to an understanding of the effects of parasitism on the host cell.Hecht is conducting research on retroviruses of hoofed animals (sheep, goats, etc.) to study the evolutionary history of the viruses. The new equipment will make it possible to explore fully most of the important molecular biology techniques necessary to characterize certain genetic sequences. The movement and transmission of particular viral sequences is being investigated to identify those viruses that are ancient and therefore found in many breeds and species versus those that are modern and still actively spreading. This approach is also valuable for better understanding the spread of other important viral diseases.NLU is the primary institution serving the Lower Mississippi Delta, one of the most impoverished economic areas in the country. One of the University is most important contributions has been to improve the situation of the local population and communities by helping to bridge cultural and economic gaps. The ability to provide modern, up-to-date research and teaching opportunities in biological sciences is crucial tomaintaining a positive impact not only for biology majors but also for many pharmacy and allied health-science students served by the department. The improved infrastructure also impacts many under-represented groups in biology. As an open admissions university, NLU serves the needs of a diverse community, thereby providing access to a higher percentage of under-represented groups than are often found at selective admissions universities.