Equipment Supplement for R35 GM144059 "Writers and Erasers of Ubiquitin Moieties in Control of Cell-to-Cell Transport in Plants"

R35 GM144059 的设备补充材料“控制植物细胞间运输的泛素部分的写入器和擦除器”

• 批准号: 10796474
• 负责人: VITALY H CITOVSKY
• 金额: $ 20.69万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-16 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10796474
• 关键词: Address; Animals; Attention; Biological Models; Cells; Data; Equipment; Image; Image Analysis; Lasers; Life Cycle Stages; Microscope; Molecular; Noise; Organism; Plants; Proteins; Regulation; Resolution; Scanning; Signal Transduction; System; Tissues; Ubiquitin; Work; confocal imaging; detection platform; improved; ubiquitin isopeptidase; ubiquitin ligase

PROJECT SUMMARY Intercellular transport is central to the life cycle of multicellular organisms, and its molecular mechanisms and their regulation have been extensively studied in different model systems, including plants and animals. Yet, one key aspect of cell-to-cell transport regulation apparently escaped attention: the potential involvement of ubiquitylation/deubiquitylation (and the corresponding ubiquitin ligases and deubiquitinases). In our parental project, we have obtained evidence for the existence of such a regulatory system and are characterizing its molecular components and mechanism(s) of action. This work heavily depends on the use of a confocal laser scanning microscope to detect and follow the fate of fluorescently tagged proteins in plant tissues. Based on the current and projected trajectory of the project, there is an urgent need for improvement of our confocal imaging quality, i.e., resolution and signal-to-noise ratio, to enhance the quality of acquired data and their interpretation. To address this critical technical aspect of the project, we propose acquiring a Zeiss Airyscan 2 detection system with a Zeiss 60A R2 offline analysis workstation that can be incorporated into our existing Zeiss LSM 900 machine and provide significant improvement to its imaging and image analysis capabilities.

项目概要 细胞间运输是多细胞生物生命周期的核心，其 分子机制及其调控已在不同模型中得到广泛研究 系统，包括植物和动物。然而，细胞间运输调节的一个关键方面 显然没有引起注意：泛素化/去泛素化的潜在参与（和 相应的泛素连接酶和去泛素酶）。在我们的父母项目中，我们有 获得了此类监管体系存在的证据，并正在描述其特征 分子成分和作用机制。 这项工作很大程度上依赖于使用共焦激光扫描显微镜来检测 并追踪植物组织中荧光标记蛋白的命运。基于当前和 该项目的预计轨迹，迫切需要改进我们的共焦 成像质量，即分辨率和信噪比，以提高采集数据的质量 以及他们的解释。为了解决该项目的这一关键技术问题，我们建议 购买带有 Zeiss 60A R2 离线分析工作站的 Zeiss Airyscan 2 检测系统 可以集成到我们现有的 Zeiss LSM 900 机器中，并提供显着的 提高其成像和图像分析能力。