Development of a Cell Culture Facility for Undergraduate Investigations in Eukaryotic Gene Expression

开发用于真核基因表达本科研究的细胞培养设施

• 批准号: 9850427
• 负责人: Cheryl Davis
• 金额: $ 2.33万
• 依托单位: Western Kentucky University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9850427&HistoricalAwards=false
• 关键词: Development; Cell; Culture; Facility; Undergraduate

The major focus of this project is an upgrade of existing laboratories for cell biology, virology and recombinant gene technology. Other courses such as animal physiology, immunology, plant physiology, and independent undergraduate research projects also benefit from this facility. With the new cell culture facility, students are able to use animal and plant cells to study cellular events and gene expression. Virology has added a laboratory component to its syllabus. Recombinant gene technology (RGT) is a specialized program; the only one of its kind in the state and one of a handful in the nation. A cell culture facility supports laboratory study of eukaryotic gene expression in the RGT course. Results are being disseminated through state and national meetings. Laboratory manuals, web-site and publications are being distributed. Current assessment tests for undergraduate biology majors and student evaluation/feedback surveys are being modified to analyze success of this project. The addition of this facility provides a greater breadth of experience for students, and especially prepares Kentucky students, who are often first generation college graduates, with the laboratory skills needed to compete for positions both within the state and across the nation.

该项目的主要重点是升级现有的细胞生物学、病毒学和重组基因技术实验室。 其他课程，如动物生理学，免疫学，植物生理学和独立的本科生研究项目也受益于这个设施。 有了新的细胞培养设施，学生能够使用动物和植物细胞来研究细胞事件和基因表达。病毒学在教学大纲中增加了实验部分。重组基因技术（RGT）是一个专门的计划，在该州只有一个，在全国少数之一。 细胞培养设施支持RGT课程中真核基因表达的实验室研究。正在通过州和国家会议传播成果。正在分发实验室手册、网站和出版物。目前正在修改本科生物专业的评估测试和学生评价/反馈调查，以分析该项目的成功。该设施的增加为学生提供了更广泛的经验，特别是准备肯塔基州的学生，谁往往是第一代大学毕业生，与所需的实验室技能竞争的位置都在国家和全国各地。