Development of dynamic hydrogel systems to tune local microenvironement in three dimensional cell culture system

开发动态水凝胶系统来调节三维细胞培养系统中的局部微环境

基本信息

批准号：
22F20708
负责人：
竹内昌治
金额：
$ 0.77万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-22 至 2023-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22F20708/
关键词：
Gold nanoparticles hydrogel controlled release DNA photothermal effect microfluidic cell culture epithelial cells

项目摘要

The focus of my research was the development of nanoparticle systems that could be introduced into commercial hydrogels to tune their properties. One type of nanoparticle that I worked with are fluorescent quantum dots. To use these particles in hydrogel systems, it is essential to optimize their stability and optical properties in physiologically relevant conditions. I conducted a fundamental study, showing how previously published articles did not rigorously investigate the surface chemistry of these particles. The results of this investigation were published in the journal ACS Applied Nano Materials and will be presented to a conference in June 2023.Another nanoparticle system that I continued developing was based on gold nanorods and DNA. Gold nanorods can very efficiently generate heat when irradiated by a red laser, and the resulting heat is transferred to the surrounding environment. When double strands of DNA are immobilized on the rods’ surface, the heat causes DNA melting and subsequent release of one strand into the surrounding media. This mechanism was confirmed using fluorophore-labelled DNA, and the plan was to use a laser for the targeted release of a growth factor in 3D cultures of endothelial cells. Endothelial cells spheroids were produced and used in a sprouting assay to test the release of growth factor from the Gold-DNA particles. More tests will be required to fully characterize the response of this system to laser irradiation and the resulting morphological changes on the endothelial cells.

我研究的重点是可以将纳米颗粒系统的开发引入商业水凝胶中以调整其性能。我与之合作的一种纳米颗粒是荧光量子点。要在水凝胶系统中使用这些颗粒，必须在物理相关条件下优化其稳定性和光学特性。我进行了一项基本研究，显示了先前发表的文章如何不严格研究这些颗粒的表面化学。该研究的结果发表在ACS应用的纳米材料杂志上，并将在2023年6月的会议上介绍。我继续开发的其他纳米颗粒系统是基于金纳米棒和DNA。当红色激光照射时，金纳米棒可以非常有效地产生热量，并且将所得的热量转移到周围环境中。当将DNA的双链固定在杆表面上时，热量会导致DNA熔化，然后将一条线释放到周围的介质中。使用荧光团标记的DNA确认了这种机制，该计划是使用激光在内皮细胞的3D培养物中靶向释放生长因子。在发芽测定法中产生并使用了内皮细胞，以测试从金-DNA颗粒中释放生长因子。需要进行更多的测试，以充分表征该系统对激光照射的响应以及内皮细胞的形态学变化。