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Development of dynamic hydrogel systems to tune local microenvironement in three dimensional cell culture system

开发动态水凝胶系统来调节三维细胞培养系统中的局部微环境

基本信息

批准号：
22F20708
负责人：
竹内昌治
金额：
$ 0.77万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-22 至 2023-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22F20708/
关键词：
Gold nanoparticles hydrogel controlled release DNA photothermal effect microfluidic cell culture epithelial cells

项目摘要

The focus of my research was the development of nanoparticle systems that could be introduced into commercial hydrogels to tune their properties. One type of nanoparticle that I worked with are fluorescent quantum dots. To use these particles in hydrogel systems, it is essential to optimize their stability and optical properties in physiologically relevant conditions. I conducted a fundamental study, showing how previously published articles did not rigorously investigate the surface chemistry of these particles. The results of this investigation were published in the journal ACS Applied Nano Materials and will be presented to a conference in June 2023.Another nanoparticle system that I continued developing was based on gold nanorods and DNA. Gold nanorods can very efficiently generate heat when irradiated by a red laser, and the resulting heat is transferred to the surrounding environment. When double strands of DNA are immobilized on the rods’ surface, the heat causes DNA melting and subsequent release of one strand into the surrounding media. This mechanism was confirmed using fluorophore-labelled DNA, and the plan was to use a laser for the targeted release of a growth factor in 3D cultures of endothelial cells. Endothelial cells spheroids were produced and used in a sprouting assay to test the release of growth factor from the Gold-DNA particles. More tests will be required to fully characterize the response of this system to laser irradiation and the resulting morphological changes on the endothelial cells.

我的研究重点是纳米粒子系统的发展，这种系统可以被引入到商业水凝胶中，以调整它们的性质。我研究的一种纳米粒子是荧光量子点。为了在水凝胶体系中使用这些颗粒，必须在生理相关条件下优化它们的稳定性和光学特性。我进行了一项基础研究，展示了以前发表的文章如何没有严格研究这些颗粒的表面化学性质。这项研究的结果发表在《ACS应用纳米材料》杂志上，并将在2023年6月的一次会议上发表。我继续开发的另一个纳米粒子系统是基于金纳米棒和DNA。当被红色激光照射时，金纳米棒可以非常有效地产生热量，并将产生的热量传递给周围的环境。当双链DNA固定在杆状细胞表面时，热量会导致DNA融化，随后其中一条链会释放到周围的介质中。利用荧光团标记的DNA证实了这一机制，并计划在内皮细胞的3D培养物中使用激光靶向释放生长因子。内皮细胞球体产生并用于发芽试验，以测试生长因子从金- dna颗粒的释放。需要更多的试验来充分表征该系统对激光照射的反应以及由此产生的内皮细胞形态学变化。