Instrumentation for a New Experimental Biochemistry Laboratory Course

新实验生物化学实验室课程的仪器

• 批准号: 9850778
• 负责人: Henry Brenner
• 金额: $ 1.77万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-15 至 2000-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9850778&HistoricalAwards=false
• 关键词: Instrumentation; New; Experimental; Biochemistry; Laboratory

This project involves undergraduate students in experiments using modern methods of analysis of biomolecules. In particular, a series of integrated experiments are being developed that assess the activity of proteins known to bind genomic DNA at specific sequences. These proteins are critical for regulating the information flow from DNA to RNA and on to polypeptides. Equipment enables students to spend more time thinking about the experimental results and analyzing the data. An example of this is the determination of protein concentrations in fractions following chromatographic separations via gel exclusion chromatographic matrices, hydrophobic interaction matrices, and affinity matrices. A method for non-radioactive, non-enzymatic detection of the activity of sequence specific DNA binding proteins is being developed that can readily be used in undergraduate teaching laboratories. A family member of the group of proteins related to the Myb oncogene binds telomeric DNA sequences specifically, and a recombinant DNA molecule are being constructed that will also allow expression, purification and analysis of its DNA binding activities. This course is geared to helping students experience first-hand the excitement of scientific inquiries and to relate their experiments with current investigations in basic biochemical and biomedical research.

这个项目让本科生参与使用现代生物分子分析方法的实验。特别是，正在开发一系列综合实验，以评估已知与基因组DNA结合在特定序列上的蛋白质的活性。这些蛋白质对于调节从DNA到RNA再到多肽的信息流是至关重要的。设备使学生能够花更多的时间思考实验结果和分析数据。这方面的一个例子是通过凝胶排阻色谱矩阵、疏水相互作用矩阵和亲和矩阵对层析分离后的级分中蛋白质浓度的测定。一种非放射性、非酶法检测序列特异性DNA结合蛋白活性的方法正在开发中，该方法可随时用于本科教学实验室。Myb癌基因相关蛋白家族中的一个成员可以与端粒DNA序列特异性结合，正在构建一种重组DNA分子，该分子也将允许表达、纯化和分析其DNA结合活性。本课程旨在帮助学生亲身体验科学探究的刺激，并将他们的实验与基础生化和生物医学研究中的当前研究联系起来。