Instrumental Electrophoresis and Chromatography in Bioanalytical Chemistry

生物分析化学中的仪器电泳和色谱法

• 批准号: 9851084
• 负责人: William Hobey
• 金额: $ 8.11万
• 依托单位: Worcester Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9851084&HistoricalAwards=false
• 关键词: Instrumental; Electrophoresis; Chromatography; Bioanalytical; Chemistry

The newly reorganized Department of Chemistry and Biochemistry at the Worcester Polytechnic Institute proposes to introduce Instrumental Electrophoresis and Chromatography in Bioanalytical Chemistry by converting a very traditional wet analytical chemistry laboratory course into a largely instrumental modern bioanalytical course. Students will be exposed to protein, nucleic acid, metabolite, and drug analysis and separation using rapid instrumental means with highly sensitive and discriminating detectors viz. capillary electrophoresis and high performance liquid chromatography, both with diode array detectors. Through use of experiments such as the identification and isolation of isozymes, the products of enzyme cleavage of proteins and genes, and the chemical modification of membrane proteins, students will be exposed to a variety of specific methodologies. A "capstone" experiment in which the student team must choose and justify the "most appropriate" approach to an analysis will test their understanding of principles. Professional reports on the specific experiments developed, and dissemination of an Instrumental Chromatography laboratory manual should aid other schools in introducing such methods, particularly capillary electrophoresis where the literature on undergraduate usage, while growing, is still sparse. These changes are aimed first at the Biochemistry majors for which the Department was given authority in September 1995, and at those Chemistry majors pursuing the recently introduced Medicinal Chemistry option. However, all Chemistry majors should benefit from the changes. Generally, the revised course will be taken in the second year, with the equipment being used in required research project work in the third and/or fourth years.

新重组的化学和生物化学系在伍斯特理工学院建议引入仪器电泳和色谱生物分析化学转换成一个非常传统的湿分析化学实验室课程，主要是仪器现代生物分析课程。学生将接触到蛋白质，核酸，代谢物和药物的分析和分离使用快速仪器手段与高灵敏度和鉴别检测器，即毛细管电泳和高效液相色谱，都与二极管阵列检测器。通过使用实验，如同工酶的鉴定和分离，蛋白质和基因的酶裂解产物，以及膜蛋白的化学修饰，学生将接触到各种具体的方法。在一个“顶点”实验中，学生团队必须选择并证明“最合适”的分析方法，这将测试他们对原理的理解。关于特定实验的专业报告，以及仪器色谱实验室手册的传播应有助于其他学校引入此类方法，特别是毛细管电泳，其中关于本科生使用的文献虽然不断增长，但仍然很少。这些变化首先针对生物化学专业，该部门于1995年9月获得授权，并在那些追求最近推出的药物化学选项的化学专业。然而，所有化学专业的学生都应该从这些变化中受益。一般来说，修订后的课程将在第二年进行，设备将在第三年和/或第四年用于所需的研究项目工作。