High-resolution mass spectrometer with Liquid Chromatography (LC) and Capillary Electrophoresis (CE)

具有液相色谱 (LC) 和毛细管电泳 (CE) 功能的高分辨率质谱仪

• 批准号: 515835726
• 金额: --
• 依托单位: Rheinische Friedrich-Wilhelms-Universität Bonn
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/515835726?language=en
• 关键词: resolution; mass; spectrometer; Liquid; Chromatography

Mass spectrometry with separation of compounds by e.g. liquid chromatography (LC-MS) has emerged as a key method for large scale metabolomic measurements. In contrast to direct infusion mass spectrometry, LC-MS offers several advantages because compounds from complex sample matrices are separated into different peaks, which reduces ion suppression and results in improved sensitivity. This proposal aims at the procurement of a new tandem mass spectrometer for LC-MS and capillary electrophoresis-mass spectrometry (CE-MS) measurements of small molecules, both employing targeted and non-targeted strategies. The new instrument will be used for the identification and quantification of signaling molecules (e.g., phosphoinositides and inositol-phosphates), secondary plant metabolites (e.g., flavonoids) and other molecules, with a focus on plants and bacteria. We will use the LC-MS to establish a pipeline for the identification of metabolites that differentially accumulate between two biological systems (non-targeted metabolomics), e.g. by comparing wild types and mutants, or organisms exposed to control or stress conditions. The instrument will be switched to CE-MS mode for the measurement of inositol-phosphates and other polar, anionic metabolites (NADPH, ATP, etc.). The CE-MS system is currently not available on campus at the University of Bonn. The mass spectrometer will be located and integrated into the existing Lipidomics/Metabolomics Platform at the Institute of Molecular Physiology and Biotechnology of Plants (IMBIO) of the University of Bonn. This platform already operates two LC-MS instruments, which are used at high capacity for direct infusion/shotgun measurements of glycerolipids, and LC-MS analysis of low abundant metabolites. The new mass spectrometer will be used for measurements with high mass accuracy and sensitivity, which is required for the identification and quantification of large numbers of metabolites in complex extracts. The instrument will be essential for several (joint) grant applications that have been funded or are under evaluation. The mass spectrometer will serve groups on campus, but it will also be open to collaborations with national and international research groups.

质谱法与液相色谱（LC-MS）分离的化合物已成为大规模代谢组学测量的关键方法。与直接输注质谱法相比，LC-MS具有几个优点，因为复杂样品基质中的化合物被分离到不同的峰，从而减少了离子抑制并提高了灵敏度。本提案旨在采购一种新的串联质谱仪，用于LC-MS和毛细管电泳-质谱（CE-MS）测量小分子，采用靶向和非靶向策略。新仪器将用于信号分子（如磷酸肌苷和磷酸肌醇）、次生植物代谢物（如类黄酮）和其他分子的鉴定和定量，重点是植物和细菌。我们将使用LC-MS建立一个管道，用于鉴定两种生物系统（非靶向代谢组学）之间差异积累的代谢物，例如通过比较野生型和突变体，或暴露于控制或应激条件下的生物体。仪器将切换到CE-MS模式，用于测量肌醇-磷酸盐和其他极性、阴离子代谢物（NADPH、ATP等）。目前波恩大学校园内还没有CE-MS系统。质谱仪将被安置在波恩大学植物分子生理学和生物技术研究所（IMBIO）现有的脂质组学/代谢组学平台上。该平台已经运行了两台LC-MS仪器，用于高容量直接输注/霰弹枪测量甘油脂，以及低丰度代谢物的LC-MS分析。新的质谱仪将用于具有高质量精度和灵敏度的测量，这是鉴定和定量复杂提取物中大量代谢物所需要的。该工具对于已获得资助或正在评估的若干（联合）赠款申请将是必不可少的。质谱仪将为校园团体服务，但它也将开放与国内和国际研究团体合作。