SBIR Phase I: In Situ Infrared Analyzer for Multiple Components in Bioreactors

SBIR 第一阶段：生物反应器中多种成分的原位红外分析仪

• 批准号: 9860867
• 负责人: James Kane
• 金额: $ 9.98万
• 依托单位: Polestar Technologies Incorporated
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9860867&HistoricalAwards=false
• 关键词: SBIR; Phase; Situ; Infrared; Analyzer

9860867 This Small Business Innovation Research (SBIR) Phase I project will develop a multi-component mid-infrared analyzer system which includes an innovative in-situ internal reflection probe with a flexible coupling to an infrared spectrometer. The probe will sample multiple components in the fermentation broth of a bioreactor via evanescent field interaction in an attenuated total reflection (ATR) crystal element. The infrared light throughout of flexible coupling elements is higher than conventional, halcogenide infrared fibers and the elements are mechanically more robust. The analyzer also contains a self-cleaning filter mechanism to prevent biofouling of the active probe surface. In the Phase I effort, the analyzer will monitor glycerol and acetate concentrations in a bioreactor system with a one minute total measurement cycle time and sensitivity down to 1 g/l range. The Infrared analyzer system will find commercial applications in bioreactor systems used in the biotechnology, pharmaceutical, and food Industries. The analyzer will provide improved process control and efficiency in these industries. The proposed in situ infrared multiple component analyzer will provide a valuable process control and diagnostics tool for use in bioreactors in the biotechnology, pharmaceutical, and food industries. It will also find applications in the petroleum and chemical feedstock Industries.

9860867 这个小型企业创新研究（SBIR）第一阶段项目将开发一个多组分中红外分析仪系统，其中包括一个创新的原位内反射探头，可灵活耦合到红外光谱仪。 该探头将通过衰减全反射（ATR）晶体元件中的倏逝场相互作用对生物反应器发酵液中的多种组分进行采样。柔性耦合元件的红外光通过量高于常规的硫族红外光纤，并且元件在机械上更坚固。分析仪还包含自清洁过滤器机制，以防止活性探针表面的生物污染。在第一阶段的工作中，分析仪将监测生物反应器系统中的甘油和乙酸盐浓度，总测量周期为1分钟，灵敏度低至1 g/l范围。 红外分析仪系统将在生物技术、制药和食品工业的生物反应器系统中找到商业应用。该分析仪将在这些行业中提供改进的过程控制和效率。 建议在原位红外多组分分析仪将提供一个有价值的过程控制和诊断工具，在生物技术，制药和食品工业的生物反应器中使用。 它还将在石油和化学原料工业中找到应用。