SBIR Phase I: Real Time Measurement of Dissolved Carbon Dioxide in the Bioreactor System

*9761352这个小型企业创新研究第一阶段项目将开发一种新型的光学传感系统，用于实时测量生物反应器系统细胞培养基中溶解的二氧化碳。生物反应器中的二氧化碳浓度是反映微生物呼吸和生物活性的重要指标，也是控制反应器中细胞代谢的重要参数。二氧化碳在生物反应器中的积累对微生物形态有多种影响，并抑制细胞生长和产物形成。由于缺乏可靠的传感设备，目前没有对生物反应器介质中溶解的二氧化碳进行监测。我们提出了一种光学传感器，它将为各种发酵过程提供快速和连续的细胞培养基中二氧化碳浓度的测定。提出的技术是基于将二氧化碳捕获在排斥液体的聚合物膜中，并在红外区域对二氧化碳的特定吸收进行光谱测量。监测反应堆中溶解二氧化碳的探测器将对生物技术和制药市场具有重大价值。人类治疗领域需要对重组细菌等材料进行严格的质量控制。由于目前还没有可用的传感器，因此开发一种溶解二氧化碳传感器非常重要。***

*** 9761352 This Small Business Innovation Research (SBIR) Phase I project will develop a novel optical sensing system for real time measurement of dissolved carbon dioxide in the cell culture medium for bioreactor systems. The concentration of carbon dioxide in the bioreactor medium is an important indicator for respiratory and bioactivity of microorganisms and a useful parameter for controlling cell metabolism in reactors. Carbon dioxide accumulation in the bioreactor medium has a variety of influences on microbial morphology, and inhibition of cell growth and product formation. With the lack of reliable sensing devices, dissolved carbon dioxide in the bioreactor medium is not currently monitored. We propose an optical sensor which will provide rapid and continuous determination of carbon dioxide concentration in the cell culture medium for various fermentation processes. The proposed technique is based on trapping carbon dioxide in a polymer membrane which repels liquid, and spectral measurement of carbon dioxide specific absorption in the infrared region. The probe to monitor dissolved carbon dioxide in reactors will have significant value to the biotechnology and pharmaceutical markets. The field of human therapeutics requires close quality control of materials such as recombinant bacteria. Development of a dissolved carbon dioxide sensor is important, since there is no sensor currently available. ***