SBIR Phase I: Real Time Measurement of Dissolved Carbon Dioxide in the Bioreactor System

* 9761352 这个小型企业创新研究（SBIR）第一阶段项目将开发一种新型的光学传感系统，用于生物反应器系统细胞培养基中溶解的二氧化碳的真实的时间测量。 生物反应器介质中二氧化碳的浓度是微生物呼吸和生物活性的重要指标，也是控制反应器中细胞代谢的有用参数。 二氧化碳在生物反应器培养基中的积累对微生物形态、细胞生长和产物形成的抑制具有多种影响。 由于缺乏可靠的传感装置，目前没有监测生物反应器培养基中溶解的二氧化碳。 我们提出了一种光学传感器，它将提供快速和连续的测定二氧化碳浓度的细胞培养基中的各种发酵过程。 所提出的技术是基于在排斥液体的聚合物膜中捕获二氧化碳，以及在红外区域中二氧化碳特定吸收的光谱测量。 用于监测反应堆中溶解二氧化碳的探头将对生物技术和制药市场具有重大价值。 人类治疗学领域需要对诸如重组细菌的材料进行严格的质量控制。 溶解二氧化碳传感器的开发是重要的，因为目前没有可用的传感器。 ***

*** 9761352 This Small Business Innovation Research (SBIR) Phase I project will develop a novel optical sensing system for real time measurement of dissolved carbon dioxide in the cell culture medium for bioreactor systems. The concentration of carbon dioxide in the bioreactor medium is an important indicator for respiratory and bioactivity of microorganisms and a useful parameter for controlling cell metabolism in reactors. Carbon dioxide accumulation in the bioreactor medium has a variety of influences on microbial morphology, and inhibition of cell growth and product formation. With the lack of reliable sensing devices, dissolved carbon dioxide in the bioreactor medium is not currently monitored. We propose an optical sensor which will provide rapid and continuous determination of carbon dioxide concentration in the cell culture medium for various fermentation processes. The proposed technique is based on trapping carbon dioxide in a polymer membrane which repels liquid, and spectral measurement of carbon dioxide specific absorption in the infrared region. The probe to monitor dissolved carbon dioxide in reactors will have significant value to the biotechnology and pharmaceutical markets. The field of human therapeutics requires close quality control of materials such as recombinant bacteria. Development of a dissolved carbon dioxide sensor is important, since there is no sensor currently available. ***