Computed Tomography Imaging Spectrometer Microscope

该项目的目标是开发一种计算机断层成像光谱仪（CTIS）显微镜，将允许快速，同时采集图像在很宽的颜色（光谱）范围。 有了这项技术，将有可能同时分析活组织内的多种细胞功能。 试图提供类似信息的当前设备相对较慢，或者只能在有限的光谱范围内提供图像数据。 该项目的范围包括开发CTIS显微镜、相关的数据采集和分析软件以及生物成像实验。在CTIS显微镜中，计算机生成全息图（CGH）分散器用于将来自对象（细胞）内每个位置的信号映射到相机阵列上的独特图案。 由于对象内的所有位置而产生的图案的总和包含关于对象的形状和颜色信息。 这些信息可以通过数学方法重建，以产生与许多独特颜色相关的物体的单个二维图像。 这就是CTIS显微镜能够同时获取空间和光谱数据的原因。 使用CTIS显微镜，可以观察不同颜色的发光探针对整个组织样本中药物或化学物质的反应，这些探针报告独特的细胞功能。这种能力提供了关于来自以异质方式响应的细胞群体的功能的生理变化的信息。 未来的应用有很多，但特别包括使用CTIS显微镜分析组织毒理学和癌细胞生长和浸润。

The objective of this project is to develop a computed-tomography imaging spectrometer (CTIS) microscope that will allow for the rapid and simultaneous acquisition of images over a wide color (spectral) range. With this technique, simultaneous analysis of multiple cell functions within living tissues will be possible. Current devices which attempt to provide similar information are relatively slow or can provide image data only over a limited spectral range. The scope of the project includes development of the CTIS microscope, the associated data-acquisition and analysis software, and biological-imaging experiments.In the CTIS microscope, a computer-generated hologram (CGH) disperser is used to map the signal from each location within an object (cells) to a distinct pattern on a camera array. The sum of patterns due to all locations within an object contains both the shape and color information about the object. This information can be mathematically reconstructed to yield individual two-dimensional images of the object associated with many unique colors. This is how the CTIS microscope is capable of acquiring spatial and spectral data simultaneously. Using the CTIS microscope, light emitting probes of different colors that report on unique cellular functions can be visualized with respect to their response to drug or chemicals throughout a tissue sample. This capability provides information about the physiological changes in function from a population of cells which respond in a heterogeneous manner. Future applications are many but in particular include use of the CTIS microscope for analysis of tissue toxicology and cancer-cell growth and infiltration.