DISSERTATION RESEARCH: The Evolution of Disease Resistance in the genus Lycopersicon

论文研究：番茄属抗病性的演变

• 批准号: 9902342
• 负责人: Charles Langley
• 金额: $ 1.01万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9902342&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Evolution; Disease; Resistance

Plant disease resistance genes encode receptors which are capable of recognizing different pathogens and signaling defense responses. A defense response triggered by the interaction between a host and pathogen limits the pathogen spread throughout the plant. The basis of disease resistance to the bacterial pathogen, Pseadomonas syringae pv tomato, has been extensively characterized at the molecular level within the host species of Lycopersicon pimpinellifolium This species is a close relative Of the edible tomato, L. esculentum, and breeding between these species has permitted the introduction of disease resistance into the crop species. Resistance is conferred by the Pto gene which encodes a receptor and signals a defense response to this bacterial pathogen. The purpose of this study is to determine whether other species of Lycopersicon are resistant to this pathogen and if resistance is conferred by the Pio gene or by different genes within these species. Functional analysis of the Pto gene has identified the regions of the gene involved in pathogen recognition and signaling of the resistance response. These data will provide a basis for analyzing natural variation in Pro genes -from both resistant and susceptible hosts. This study will investigate the taxonomic breadth of disease resistance conferred by the Pio gene and also identify how amino acid sequence differences in this gene influence disease resistance in these species.

植物抗病基因编码的受体能够识别不同的病原菌，并发出防御反应的信号。由寄主和病原菌的相互作用触发的防御反应限制了病原菌在植物中的传播。番茄假单胞菌(Pseadomonas Syringae PV)对番茄细菌性病原菌的抗病基础已在分子水平上得到了广泛的研究。番茄寄主与食用番茄L.esculentum是近亲关系，这些物种之间的育种使抗病基因得以引入作物物种。抗性是由PTO基因赋予的，该基因编码一种受体，并发出对这种细菌病原体的防御反应。这项研究的目的是确定其他种类的番茄是否对这种病原菌具有抗性，以及抗性是由Pio基因还是由这些物种中的不同基因赋予的。对PTO基因的功能分析已经确定了参与病原菌识别和抗性反应信号传递的基因区域。这些数据将为分析抗病和感病宿主Pro基因的自然变异提供基础。这项研究将调查Pio基因赋予抗病的分类广度，并确定该基因中的氨基酸序列差异如何影响这些物种的抗病能力。