Association Genetics of Natural Genetic Variation and Complex Traits in Pine

松树自然遗传变异与复杂性状的关联遗传学

• 批准号: 0501763
• 负责人: Charles Langley
• 金额: $ 674.5万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0501763&HistoricalAwards=false
• 关键词: Association; Genetics; Natural; Genetic; Variation

The long term goal is to genetically dissect complex traits and understand the relationship between naturally occurring genetic and phenotypic variation in forest trees. In this project the relationships between naturally occurring genetic and phenotypic variation in Pinus taeda L. will be identified. Loblolly pine, a gymnosperm, is well-positioned phylogenetically to help in understanding the evolution of adaptive traits in land plants. Specific objectives are to (1) identify all common variants in 5000 genes, creating a first-of-its-kind community resource of genomic sequence for loblolly pine, and (2) associate genetic variation at 1000 loci with traits of economic importance. The traits will include chemical and anatomical wood properties and resistance to disease. All DNA sequence data will be submitted immediately to public access databases, GenBank and TreeGenes (dendrome.ucdavis.edu). Genotypic and phenotypic data will be immediately deposited into TreeGenes. The overarching goal of community coalescence will be accomplished by integrating loblolly pine genomics and bioinformatics efforts across the United States and making them available through the public databases. Identification of specific genes controlling naturally occurring phenotypic variation will provide 1) a greater understanding of trait inheritance in plants, 2) an indirect selection tool for tree breeders, and 3) a set of potentially useful genetic reagents for genetic modification. By aggressively seeking to identify most of the major genes controlling specific wood property and disease related traits in loblolly pine, a significant breakthrough is anticipated in the understanding of the genetics of complex traits and the potential economic impact of indirect selection. Marker-aided-selection based on desired variants in genes controlling economic traits can be used across pedigrees within both breeding and natural populations. Such a tool would have immediate and beneficial ramifications for applied tree improvement programs by dramatically reducing testing costs and breeding cycle times. Ultimately, such information could be useful in guiding gene conservation efforts and enhancing our ability to cope with the growing challenges of climate change, shifting habitats and evolving pest populations.

长期目标是从遗传上剖析复杂的性状，并了解林木中自然发生的遗传和表型变异之间的关系。 本研究旨在探讨火炬松天然遗传变异与表型变异之间的关系。将被识别。 火炬松是一种裸子植物，在植物遗传学上处于有利地位，有助于理解陆地植物适应性特征的进化。具体目标是（1）确定5000个基因中的所有常见变异，创建火炬松基因组序列的首个社区资源，以及（2）将1000个基因座的遗传变异与经济重要性性状相关联。这些特性将包括木材的化学和解剖特性以及抗病性。所有DNA序列数据将立即提交给公共访问数据库、GenBank和TreeGenes（dendrome.ucdavis.edu）。 基因型和表型数据将立即存入TreeGenes。社区联合的总体目标将通过整合美国各地的火炬松基因组学和生物信息学工作并通过公共数据库提供来实现。 控制自然发生的表型变异的特定基因的鉴定将提供1）植物性状遗传的更好理解，2）树木育种者的间接选择工具，和3）一组用于遗传修饰的潜在有用的遗传试剂。 通过对火炬松木材性状和病害相关性状主效基因的研究，有望在理解复杂性状的遗传学和间接选择的潜在经济影响方面取得重大突破。 基于控制经济性状的基因中的期望变体的标记辅助选择可以在育种群体和自然群体内跨谱系使用。 这样的工具将通过显著降低测试成本和育种周期时间，对应用树木改良计划产生直接和有益的影响。 最终，这些信息可能有助于指导基因保护工作，提高我们科普气候变化、生境变化和害虫种群演变等日益严峻的挑战的能力。