Group Research: Engineering Plant One-Carbon Metabolism

课题组研究：工程植物一碳代谢

• 批准号: 9902877
• 负责人: Hans Bohnert
• 金额: $ 13.2万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9902877&HistoricalAwards=false
• 关键词: Group; Research; Engineering; Plant; Carbon

Primary and secondary metabolism intersect in the one-carbon (1-c) area with primary metabolism supplying most of the 1-c units and competing with secondary metabolism for their use. This competition is potentially severe because plant secondary metabolism imposes hugh demands for 1-c units, especially methyl groups for the synthesis of lignin, alkaloids and osmoprotectant compounds such as glycine betaine and D-ononitol. As many current metabolic engineering projects aim to change levels of these products, and hence methyl group demand, it is essential that one understands how 1-c metabolism is regulated so that one can successfully engineer the 1-c supply to match demand. To do this requires skills in molecular biology, metabolic and analytical chemistry, in vivo NMR, and computer modeling. These skills will come from five Principal Investigators (PIs) with complementary and overlapping expertise. All PIs are used to doing interdisciplinary teamwork.

初级代谢和次级代谢在一碳(1-c)区域相交，初级代谢提供大部分1-c单位，并与次级新陈代谢竞争使用它们。这种竞争可能是激烈的，因为植物的次生代谢要求Hugh需要1-c单位，特别是用于合成木质素、生物碱和渗透保护剂化合物(如甘氨酸甜菜碱和D-月桂醇)的甲基。由于目前许多代谢工程项目的目标是改变这些产品的水平，从而改变甲基团的需求，因此了解1-C代谢是如何调节的是至关重要的，以便能够成功地设计1-C供应以匹配需求。要做到这一点，需要掌握分子生物学、代谢和分析化学、活体核磁共振和计算机建模方面的技能。这些技能将来自五个具有互补和重叠专业知识的首席调查员(PI)。所有的PI都习惯于进行跨学科的团队合作。