SGER: System for Multidimensional Proteome Analysis

SGER：多维蛋白质组分析系统

• 批准号: 9906662
• 负责人: Daniel Knapp
• 金额: $ 9.99万
• 依托单位: Medical University of South Carolina
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9906662&HistoricalAwards=false
• 关键词: SGER; System; Multidimensional; Proteome; Analysis

9906662Abstract Following the complete sequencing of the genome, the next challenge will be to describe the repertoire of proteins synthesized by specific cell types under specific circumstances, i.e. proteome analysis. Such study will require development of analytical methodology capable of addressing very complex mixtures of proteins. The proposed work is directed toward development of such methodology using an integrated microfluidics (microchip) system employing three orthogonal separation/analysis modes (isoelectric focusing, partition chromatography and mass spectrometry) followed by computer analysis of the resulting data. The proposed system will be capable of identification of proteins in complex mixtures, including post-translationally modified proteins and definition of quantitative differences between protein mixtures. The prototype of the proposed system will be implemented using recently developed soft-lithography techniques.

9906662摘要在完成基因组测序之后，下一个挑战将是描述特定细胞类型在特定环境下合成的蛋白质组，即蛋白质组分析。 这种研究将需要开发能够处理非常复杂的蛋白质混合物的分析方法。 建议的工作是针对开发这样的方法，使用集成微流体（微芯片）系统采用三个正交的分离/分析模式（等电聚焦，分配色谱和质谱），然后通过计算机分析得到的数据。 所提出的系统将能够识别复杂混合物中的蛋白质，包括后修饰的蛋白质和蛋白质混合物之间的定量差异的定义。 该系统的原型将使用最近开发的软光刻技术。