HPLC-Quadrupole Ion Trap Mass Spectrometer for Protein and Peptide Analysis

申请资金用于购买Finnigan-MAT LCQ离子阱质谱仪和相关的Hewlett Packard HPLC，供来自南卡罗来纳州医科大学三个不同基础科学系（生物化学、药理学和细胞生物学）的七名独立、由联邦政府资助的研究人员使用。建议在胰岛素样蛋白、视网膜色素和结合蛋白、GTP结合蛋白、精子运动蛋白、透镜球状蛋白（晶状体蛋白）和regA蛋白的分离、纯化和合成中使用这种高灵敏度、有效的设备。 还简要介绍了也将从拟议的仪器中受益的另外六个用户的研究项目。使用LCQMS仪器的初步数据显示，一个透镜蛋白证明LCQ技术不仅提供了最先进的灵敏度，但节省了大量的时间和样品处理，通过其能力进行在线HPLC-MS和HPLC-MS/MS的一个色谱运行。 拟议的仪器将成为MUSC质谱设施的一部分，该设施已发展成为一个主要致力于研究蛋白质结构和功能的实验室。它结合了质谱的新发展和应用研究，该技术应用于与该设施相关的教师和学生的重要研究问题，并为一般MUSC社区以及国家和国际合作者提供服务。

Funds are requested to purchase a Finnigan-MAT LCQ Ion Trap Mass Spectrometer and associated Hewlett Packard HPLC for use by a group of seven independent, federally funded investigators from three different basic science departments (biochemistry, pharmacology, and cell biology) at the Medical University of South Carolina. It is proposed to use this highly sensitive, efficient equipment in the isolation, purification, and synthesis of insulin-like proteins, retinal pigments and binding proteins, GTP-binding proteins, sperm motility proteins, lens globular proteins (crystallins), and regA proteins. The research projects of six additional users who will also benefit from the proposed instrument are also briefly described. Preliminary data using the LCQMS instrument are presented for a lens protein demonstrating that the LCQ technology provides not only state-of-the-art sensitivity, but saves enormous amounts of time and sample handling through its capability to perform on-line HPLC-MS and HPLC-MS/MS on one chromatographic run. The proposed instrument will become a part of MUSC's Mass Spectrometry Facility which has evolved into a laboratory primarily devoted to the study of protein structure and function. It combines research in new developments and applications of mass spectrometry, application of this technology to important research problems by the faculty and students associated with the facility, and service to the general MUSC community as well as to national and international collaborators.