HPLC-Quadrupole Ion Trap Mass Spectrometer for Protein and Peptide Analysis

申请资金用于购买Finnigan-MAT LCQ离子陷阱质谱仪和相关的惠普高效液相色谱仪，供来自南卡罗来纳医科大学三个不同基础科学系(生物化学、药理学和细胞生物学)的七名独立、联邦资助的研究人员使用。建议使用这种高灵敏、高效的设备来分离、纯化和合成胰岛素样蛋白、视网膜色素和结合蛋白、GTP结合蛋白、精子运动蛋白、晶状体球蛋白(晶体蛋白)和RegA蛋白。还简要介绍了另外六个用户的研究项目，这些用户也将受益于拟议的文书。使用LC QMS仪器对一种晶状体蛋白质的初步数据表明，LC Q技术不仅提供了最先进的灵敏度，而且由于其能够在一次色谱运行中进行在线LC-MS和LC-MS/MS，因此节省了大量的时间和样品处理。拟议中的仪器将成为南加州大学质谱学设施的一部分，该设施已演变为主要致力于研究蛋白质结构和功能的实验室。它结合了对质谱学的新发展和应用的研究，由与该设施相关的教职员工和学生将这项技术应用于重要的研究问题，以及为一般MUSC社区以及国内和国际合作者提供服务。

Funds are requested to purchase a Finnigan-MAT LCQ Ion Trap Mass Spectrometer and associated Hewlett Packard HPLC for use by a group of seven independent, federally funded investigators from three different basic science departments (biochemistry, pharmacology, and cell biology) at the Medical University of South Carolina. It is proposed to use this highly sensitive, efficient equipment in the isolation, purification, and synthesis of insulin-like proteins, retinal pigments and binding proteins, GTP-binding proteins, sperm motility proteins, lens globular proteins (crystallins), and regA proteins. The research projects of six additional users who will also benefit from the proposed instrument are also briefly described. Preliminary data using the LCQMS instrument are presented for a lens protein demonstrating that the LCQ technology provides not only state-of-the-art sensitivity, but saves enormous amounts of time and sample handling through its capability to perform on-line HPLC-MS and HPLC-MS/MS on one chromatographic run. The proposed instrument will become a part of MUSC's Mass Spectrometry Facility which has evolved into a laboratory primarily devoted to the study of protein structure and function. It combines research in new developments and applications of mass spectrometry, application of this technology to important research problems by the faculty and students associated with the facility, and service to the general MUSC community as well as to national and international collaborators.