Elucidating Structure Versus Function Relationships for Adsorbed Enzyme Layers

本项目的目的是利用一系列实验工具，以综合的方式研究吸附酶的结构-功能关系。其中包括一套互补的、非侵入性的光谱和成像工具，用于定量分析表征良好的表面上的吸附层结构。此外，还将采用一种新的无创测定方法，在完全相同类型的表面上测量酶催化反应的速率。需要确定的量包括吸附等温线和动力学、单个分子和吸附层的结构信息、活性位点可及性和酶活性。

The objective of this project is to investigate structure-function relationships for adsorbed enzymes in an integrated manner using a battery of experimental tools. These include a set of complementary, non-invasive spectroscopic and imaging tools to quantitatively analyze the adsorbed layer structure on well-characterized surfaces. Also, a new assay to non-invasively measure the rates of enzyme-catalyzed reactions on exactly the same types of surfaces would be used. Quantities to be determined include adsorption isotherms and kinetics, structural information on both individual molecules and the adsorbed layer, active site accessibility, and enzyme activity.