Elucidating Structure Versus Function Relationships for Adsorbed Enzyme Layers

阐明吸附酶层的结构与功能关系

• 批准号: 9907504
• 负责人: Robert Tilton
• 金额: $ 49.94万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2004-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9907504&HistoricalAwards=false
• 关键词: Elucidating; Structure; Versus; Function; Relationships

The objective of this project is to investigate structure-function relationships for adsorbed enzymes in an integrated manner using a battery of experimental tools. These include a set of complementary, non-invasive spectroscopic and imaging tools to quantitatively analyze the adsorbed layer structure on well-characterized surfaces. Also, a new assay to non-invasively measure the rates of enzyme-catalyzed reactions on exactly the same types of surfaces would be used. Quantities to be determined include adsorption isotherms and kinetics, structural information on both individual molecules and the adsorbed layer, active site accessibility, and enzyme activity.

这个项目的目标是利用一系列实验工具以综合的方式研究吸附的酶的结构-功能关系。其中包括一套补充的、非侵入性的光谱和成像工具，用于定量分析表征良好的表面上的吸附层结构。此外，还将使用一种新的测试方法，非侵入性地测量在完全相同类型的表面上酶催化反应的速度。待测定的量包括吸附等温线和动力学、单个分子和吸附层的结构信息、活性位置可及性和酶活性。