SGER: Development of Molecular-Resolution Imaging of Cell-Surface Nanostructures in their Native Hydrated State
SGER: Development of Molecular-Resolution Imaging of Cell-Surface Nanostructures in their Native Hydrated State
批准号:
9908864
负责人:
Sanjoy Banerjee
金额:
$8.05万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-08-15 至 2001-01-31
中文摘要
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英文摘要
AbstractCTS-9908864S. Banerjee and R. Lal, UCSBThe investigation aims to uncover basic mechanisms by which biological flows interact with nanostructures on cell surfaces. Atomic force microscope (AFM) scanning and subnanometer-scale imaging of cell surface mechano-receptors will be performed. Those receptors are nanostructures that undergo three-dimensional molecular conformation changes under the influence of flow forces and induce cascades of biological responses that affect cell and tissue behavior. This project is focused on direct molecular visualization of nanostructures on cell surfaces by using a specially modified AFM immersed in an aqueous medium. Optical fluorescence will be used to image the same area measured with AFM. The AFM measurements will be combined with electrophisiological, immunological and biochemical techniques for structure-function correlation studies. The necessary AFM modifications for soft tissues and its accuracy limits will be investigated. The capability of the modified AFM to identify subsets of macromolecules in a complex specimen such as a cell membrane will be explored. If successful, the proposed imaging system will allow improvements in the accuracy of AFM on soft tissues immersed in liquids, with relevance to quantifying and understanding cell-flow interaction and related electrophysiological and biochemical responses. ***
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Gas-Liquid Interfaces: Turbulence and Scalar Exchange
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批准号:0941804
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项目类别:Continuing Grant
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资助金额:$0.0万
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财政年份:2009
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负责人:Sanjoy Banerjee
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依托单位:
Gas-Liquid Interfaces: Turbulence and Scalar Exchange
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批准号:0553333
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项目类别:Continuing Grant
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资助金额:$0.0万
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财政年份:2006
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负责人:Sanjoy Banerjee
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依托单位:
Development of a Technique for Nanoscale Resolution of Fluid Velocity Fields with Emphasis on Flows Over Cell-Surface Nanostructures
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批准号:0130506
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项目类别:Standard Grant
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资助金额:$8.78万
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财政年份:2002
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负责人:Sanjoy Banerjee
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依托单位:
Support Funding for the First International Symposium on Turbulence and Shear Flow Phenomena, Santa Barbara, September, 1999
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批准号:9813816
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项目类别:Standard Grant
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资助金额:$0.5万
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财政年份:1998
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负责人:Sanjoy Banerjee
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依托单位:
Investigation of Particle-Turbulence Interactions Near Boundaries By Direct Numerical Simulation
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批准号:9123297
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项目类别:Continuing Grant
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资助金额:$24.0万
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财政年份:1992
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负责人:Sanjoy Banerjee
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依托单位:
Industry/Unversity Cooperative Research Activity: Investigation of One Dimensional Wave Phenomena Two-Phase Flow
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批准号:8112667
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项目类别:Continuing Grant
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资助金额:$17.9万
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财政年份:1982
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负责人:Sanjoy Banerjee
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依托单位:
国内基金
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批准号:32070202
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项目类别:面上项目
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资助金额:58.0万元
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批准年份:2020
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负责人:汪泉
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依托单位:
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批准号:--
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项目类别:--
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资助金额:40万元
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批准年份:2020
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负责人:Vikrant Gupta
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依托单位: