SGER: Development of Molecular-Resolution Imaging of Cell-Surface Nanostructures in their Native Hydrated State

SGER：天然水合状态的细胞表面纳米结构的分子分辨率成像的发展

• 批准号: 9908864
• 负责人: Sanjoy Banerjee
• 金额: $ 8.05万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2001-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9908864&HistoricalAwards=false
• 关键词: SGER; Development; Molecular; Resolution; Imaging

AbstractCTS-9908864S. Banerjee and R. Lal, UCSBThe investigation aims to uncover basic mechanisms by which biological flows interact with nanostructures on cell surfaces. Atomic force microscope (AFM) scanning and subnanometer-scale imaging of cell surface mechano-receptors will be performed. Those receptors are nanostructures that undergo three-dimensional molecular conformation changes under the influence of flow forces and induce cascades of biological responses that affect cell and tissue behavior. This project is focused on direct molecular visualization of nanostructures on cell surfaces by using a specially modified AFM immersed in an aqueous medium. Optical fluorescence will be used to image the same area measured with AFM. The AFM measurements will be combined with electrophisiological, immunological and biochemical techniques for structure-function correlation studies. The necessary AFM modifications for soft tissues and its accuracy limits will be investigated. The capability of the modified AFM to identify subsets of macromolecules in a complex specimen such as a cell membrane will be explored. If successful, the proposed imaging system will allow improvements in the accuracy of AFM on soft tissues immersed in liquids, with relevance to quantifying and understanding cell-flow interaction and related electrophysiological and biochemical responses. ***

摘要CTS-9908864 S。Banerjee和R.这项研究旨在揭示生物流动与细胞表面纳米结构相互作用的基本机制。将对细胞表面机械受体进行原子力显微镜（AFM）扫描和亚纳米级成像。 这些受体是在流动力的影响下经历三维分子构象变化并诱导影响细胞和组织行为的生物反应级联的纳米结构。 该项目的重点是通过使用浸入水介质中的特别修改的AFM对细胞表面上的纳米结构进行直接分子可视化。 光学荧光将用于成像与AFM测量的相同区域。 AFM测量将与电生理学、免疫学和生物化学技术相结合，用于结构-功能相关性研究。 将研究用于软组织的必要的AFM修改及其准确度限制。 修改后的原子力显微镜，以确定在一个复杂的标本，如细胞膜的大分子的子集的能力将进行探讨。 如果成功的话，所提出的成像系统将允许提高AFM对浸入液体中的软组织的准确性，从而量化和理解细胞流相互作用以及相关的电生理和生化反应。 ***