C-Fern (Ceratopteris) Educational Materials Development: Sporophytic and Genetic Applications

C-Fern（Ceratopteris）教材开发：孢子体和遗传应用

• 批准号: 9950522
• 负责人: Leslie Hickok
• 金额: $ 31万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9950522&HistoricalAwards=false
• 关键词: Fern; Ceratopteris; Educational; Materials; Development

This project significantly expands the utility of educational materials based on the C-Fem (Ceratopteris) system. Much needed enhancements of the components and capabilities of CFem, especially in the saprophytic phase of the life cycle, will increase its accessibility and functionality in a wide range of educational settings. At the end of the requested funding period, C-Fem will be a mature and substantially self-generating system that will provide a unique and significant component to the biological curriculum. C-Fem is user-friendly, inexpensive and broadly applicable in a number of different educational settings. Rather than being just another life cycle, this system can illustrate in vivid ways a variety of biological principles. The distinctive sporophytic and gametophytic generations along with swimming sperm, pheromonal control of distinct sexual types, a short life cycle, development from a single cell and a wide variety of mutant stocks will combine to make this a versatile tool in the classroom or for independent research. It is especially well suited for inquiry applications and provides unique opportunities for adaptation in the genetics laboratory. There are two major materials development initiatives associated with this proposal: 1) Large-scale mutagenesis and selection in the sporophyte generation in order to obtain mutations that show significant time reductions in the sporophytic phase of the life cycle. 2) Expansion of the genetic applications of C-Fem through the generation of mono- and dihybrid mutant lines and the incorporation of sporophytic improvements from the first initiative. With these improvements, C-Fem is poised to become a principal organism in the biology classroom.

该项目大大扩展了基于C-Fem（水蕨属）系统的教材的实用性。急需加强CFEM的组成部分和能力，特别是在生命周期的卫生阶段，这将增加其在各种教育环境中的可及性和功能。在所要求的资助期结束时，C-Fem将成为一个成熟的、基本上自我生成的系统，为生物课程提供一个独特而重要的组成部分。C-Fem用户友好，价格低廉，广泛适用于许多不同的教育环境。这个系统不仅仅是另一个生命周期，它可以生动地说明各种生物学原理。独特的孢子体和配子体世代沿着游动的精子、不同性类型的信息素控制、短的生命周期、从单细胞发育和各种各样的突变株将联合收割机结合起来，使其成为课堂或独立研究中的多功能工具。它特别适合调查应用，并为遗传学实验室的适应提供了独特的机会。与该提案相关的主要材料开发计划有两个：1）在孢子体世代中进行大规模诱变和选择，以获得在生命周期的孢子体阶段显着缩短时间的突变。2)通过单杂交和双杂交突变株系的产生以及从第一个倡议开始的孢子体改进的并入，扩大C-Fem的遗传应用。随着这些改进，C-Fem有望成为生物课堂上的主要有机体。