POWRE: Model Experiments for Probing the Spreading Behavior of Exogenous Surfactant in Pulmonary Airways

摘要-9973538 S。Torian/Princeton U.致力于理解表面活性剂单层在薄液体膜上铺展的动力学或非平衡行为的大部分努力都落在了理论模型的一边。 尽管越来越多的注意力支付给这个问题的理论方面，模型还没有得到任何详细的experimental.This建议概述了实验研究与真实的肺表面活性剂的液体支持的紧密近似的选区和组成的内衬肺泡囊。 表面活性剂溶液是市售的，代表临床研究中使用的天然和合成表面活性剂混合物。 将使用区域宽折射莫尔形貌来测量扩展事件期间的时变表面斜率。 根据该数据，可以重建整个时变膜形状和扩展前沿的速度。 特别是，将量化靠近表面活性剂源的变薄速率，该区域非常容易发生膜破裂和动脉扩张模式的形成。 从理论上考虑，影响扩散过程的大多数变量是未知的。 在实验上，计划将膜形状和表面活性剂速度之间的关系量化为这些变量的函数。 还计划集中研究初始剪切应力的作用，它控制膜变薄的程度，从而控制实际输送到远端区域的表面活性剂的质量。POWRE计划将提供为期12个月的关键资金，以进行该领域的第一批铺展实验。 由于主要研究者（PI）缺乏生物医学工程方面的正规培训，因此实际上不可能从生物医学界获得此类研究的资金。 鉴于在制定扩散动力学的数学框架方面取得的成功，以及先前使用莫尔拓扑学、激光阴影术和激光干涉测量法进行实验研究的经验，PI相信一次性注入的资金可以启动这一实验计划。 这笔资金将有助于这些研究在生物医学文献中发表，这是PI的一个新领域。 这些结果有望说服生物医学界，PI在肺表面活性物质扩散领域知识渊博，她在流体物理学方面的培训可以为这一重要问题做出重大贡献。

ABSTRACTCTS-9973538S. Torian/Princeton U.Much of the effort devoted to understanding the dynamics or no-equilibrium behavior of a surfactant monolayer spreading on thin liquids films has fallen on the side of theoretical models. Despite the increasing attention paid to the theoretical aspects of this problem, the models have yet to be tested in any detail experimentally.This proposal outlines experimental studies with real lung surfactants on a liquid support the closely approximates the constituency and composition of the lining in the alveolar sacs. The surfactant solutions are commercially available and represent both natural andSynthetic surfactant mixtures used in clinical studies. Area wide refractive Moire topography will be used to measure the time variant surface slope during the spreading event. From this data the entire time variant film shape and the speed of the spreading front can be reconstructed. In particular, the rate of thinning close to the surfactant source, a region which is highly susceptible to film rupture and the formation of arterial spreading patterns, will be quantified. From theoretical considerations, most of the variables affecting the spreading process are unknown. Experimentally, it is planned to quantify the relationship between the film shape and surfactant speed as a function of these variables. It is also planned to concentrate on the role of the initial shear stress, which controls the degree of film thinning and therefore the mass of surfactant actually delivered to distal regions.The POWRE, program will provide critical funding for a 12 month period to conduct the first set of spreading experiments in this field. Given the principal investigator's (PI's) lack of formal training in biomedical engineering, it has been practically impossible to secure funding for these type of studies from the biomedical community. Given the success in formulating the mathematical framework for the spreading dynamics and given the previous experience in carrying out experimental studies using Moire topology, laser shadowgraphy and laser interferometry, the PI is confident that a one time infusion of funds can launch this experimental program. This funding will help run these studies to be published in the biomedical literature, which is a new area for the (PI). These results will hopefully convince the biomedical community that the PI is knowledgeable in the area of lung surfactant spreading and that her training in fluid physics can contribute significantly to this important problem.