POWRE: Studies of Model Compounds for the Active Site of DMSO Reductase

POWRE：DMSO 还原酶活性位点模型化合物的研究

• 批准号: 9973673
• 负责人: Sharon Burgmayer
• 金额: $ 7.5万
• 依托单位: Bryn Mawr College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-15 至 2001-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9973673&HistoricalAwards=false
• 关键词: POWRE; Studies; Model; Compounds; Active

This research is aimed at understanding the structures, spectroscopic properties, and redox behavior of Mo containing DMSOR (dimethylsulfoxide reductase) enzymes. The approach will be to synthesize model compounds which contain Mo with an inner coordination sphere similar to that postulated for the natural enzymes from Rhodobacter sphaeroides and R. capsulatus, based on x-ray crystal structures. The new compounds, of the type OMo(S2C2RR")2, will be characterized by a variety of techniques including cyclic voltammetry, NMR, FT-IR, UV-VIS, MS, ESEEM, EPR, PES, XPS, and CCD x-ray diffraction.With this POWRE award, the Chemistry Division is supporting the work of Sharon N. Burgmayer in the Department of Chemistry at Bryn Mawr College in Bryn Mawr, PA. The research is being done to understand behavior and spectroscopic properties of dimethylsuloxide reductase enzymes which contain molybdenum as the central metal. Genetic malfunctions of molybdenum enzymes in humans are the cause of the fatal combined oxidase deficiency disease. The work is expected to provide spectroscopic and structural benchmarks to aid in the interpretation of analogous data on the enzymes, and to provide insights into their active site chemistry. It is also expected to provide excellent training for undergraduates at the interface between chemistry and biochemistry, and to motivate female students to go on to careers in research.

这项研究旨在了解含有DMSOR（二甲基亚硫化还原酶）酶的MO的结构，光谱特性和氧化还原行为。 该方法将是合成具有基于X射线晶体结构的Rhodobacter sphaeroides和R. capsulatus的内部配位球的模型化合物。 OMO型（S2C2RR“）2的新化合物将以各种技术为特征宾夕法尼亚州Bryn Mawr的Bryn Mawr学院是为了了解二甲基硫氧化物还原酶的光谱和光谱酶，这些酶含有钼酶的遗传酶，这些酶是人类中造成致命的氧化疾病的原因。对酶的类似数据进行解释，并提供对其活跃部位化学的见解。