Cloning, Sequencing and Expression of a Steroid Membrane Receptor

类固醇膜受体的克隆、测序和表达

• 批准号: 9980353
• 负责人: Peter Thomas
• 金额: $ 8万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9980353&HistoricalAwards=false
• 关键词: Cloning; Sequencing; Expression; Steroid; Membrane

Steroid hormones elicit responses in many target tissues by binding to specific receptors on the cell surface. However, lack of information on the structures of these steroid membrane receptors and their genes has prevented significant progress in our understanding of this mechanism of steroid hormone action. Recently a novel gene has been identified in spotted seatrout ovaries which appears to have the characteristics of an estrogen membrane receptor. The goal of this project is to complete the characterization of this putative estrogen membrane receptor, including its binding to steroids, localization in estrogen target tissues, activation of cellular responses, and regulation. This is a high risk project because it will not be known if the novel seatrout gene is a functional estrogen membrane receptor until all the research objectives have been completed. It is probable that a knowledge of the structure of the seatrout estrogen membrane receptor can be used to identify steroid membrane receptors in other species, including humans. Therefore the identification of the first steroid membrane receptor gene and knowledge of its structure is likely to have a major impact in many areas of biology and medicine including endocrinology, psychiatry, immunology, cancer, and reproductive disorders.

类固醇激素通过与细胞表面上的特异性受体结合而在许多靶组织中引起反应。 然而，缺乏这些类固醇膜受体及其基因的结构信息，阻碍了我们对类固醇激素作用机制的理解取得重大进展。 最近，在斑点卵巢中发现了一个新的基因，它似乎具有雌激素膜受体的特征。 该项目的目标是完成这种推定的雌激素膜受体的表征，包括其与类固醇的结合，在雌激素靶组织中的定位，细胞反应的激活和调节。 这是一个高风险的项目，因为直到所有的研究目标都完成之前，人们还不知道新的seatrout基因是否是一种功能性雌激素膜受体。 这是可能的，一个结构的seatrout雌激素膜受体的知识可以用来确定类固醇膜受体在其他物种，包括人类。 因此，第一个类固醇膜受体基因的鉴定及其结构的知识可能会在生物学和医学的许多领域产生重大影响，包括内分泌学，精神病学，免疫学，癌症和生殖疾病。