Collaborative Research: Species Delimitation and Phylogenetic Relationships within Isoetes (Isoetaceae) Using Multiple Data Sets

合作研究：使用多个数据集进行水韭科 (Isoetaceae) 的物种界定和系统发育关系

• 批准号: 9981460
• 负责人: Sara Hoot
• 金额: $ 20万
• 依托单位: University of Wisconsin-Milwaukee
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-15 至 2004-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9981460&HistoricalAwards=false
• 关键词: Collaborative; Research; Species; Delimitation; Phylogenetic

9981460HootSpecies Delimitation and Phylogenetic Relationships within Isoetes UsingMultiple Data SetsIsoetes are a genus of nonflowering plants which were widespread during ancient times (Paleozoic). They have a worldwide distribution and diverse ecological adaptations. Isoetes plants may be found in streams , lakes, and ephemeral pools. The form of the plant is very simple, consisting of narrow, elongated leaves which produce spores at their base and a swollen root stock. This simple form (morphology) provides few characters, making the identification of species and determination of species relationships difficult. In addition, Isoetes species readily hybridize with each other, further confusing our understanding of species limits and relationships.This project has three main objectives: 1) to provide an evolutionary tree(phylogeny) for the genus throughout the world, 2) to identify and delimitspecies within Isoetes, and 3) to identify the original parents involved inthe formation of hybrid species. Since morphology provides few characters for this group, additional molecular data in the form of DNA sequences will be collected. In the past ten years, DNA sequence data have been an invaluable tool, helping scientists understand evolutionary relationships in a wide varietyof organisms. To better understand relationships of Isoetes species worldwide, we will use two DNA regions, one located in the chloroplast genome (atpB/rbcL intergenic spacer region) and one in the nucleus (internal transcribed spacer regions - ITS). To define species and determine the origin of hybrid species, we will use a highly variable region within a gene involved in plant reproduction (LEAFY second intron).This work is a collaborative effort between Drs. Sara B. Hoot (Universityof Wisconsin-Milwaukee) and W. Carl Taylor (Milwaukee Public Museum). S.Hoot will be largely responsible for the molecular work, C. Taylor will beinvolved in plant collection (taking him to distant sites in South America,Africa and other continents) and morphological studies of Isoetes.

9981460HootSpecies的划分和系统发育关系的研究利用多重数据集等温植物是一种不开花的植物，在古代(古生代)广泛分布。它们分布在世界各地，适应不同的生态环境。可以在溪流、湖泊和短暂的池塘中发现等温植物。植物的形状非常简单，由细长的窄叶和膨大的根茎组成，叶在底部产生孢子。这种简单的形态(形态)提供的特征很少，这使得物种的鉴定和物种关系的确定变得困难。此外，等丝虫很容易相互杂交，进一步混淆了我们对物种界限和关系的理解。这个项目有三个主要目标：1)为世界各地的属提供一个进化树(系统发生学)；2)鉴定和界定等丝虫内的物种；以及3)识别参与杂交物种形成的原始亲本。由于形态为这一组提供的特征很少，因此将收集DNA序列形式的额外分子数据。在过去的十年里，DNA序列数据一直是一个无价的工具，帮助科学家了解各种生物的进化关系。为了更好地了解世界各地等温线虫物种的关系，我们将使用两个DNA区域，一个位于叶绿体基因组(atpB/rbcL基因间隔区)，另一个位于细胞核(内部转录间隔区-ITS)。为了定义物种和确定杂交物种的起源，我们将使用与植物繁殖有关的基因中的高度可变区域(叶子第二内含子)。这项工作是威斯康星大学密尔沃基分校的Sara B.Hoot博士和密尔沃基公共博物馆的W.Carl Taylor博士共同努力的结果。S.Hoot将主要负责分子工作，C.Taylor将参与植物采集(带他去南美洲、非洲和其他大陆的遥远地点)和等丝虫的形态研究。