Genetics of Hybrid Incompatibility in Nasonia

Nasonia 杂种不亲和性的遗传学

• 批准号: 9981634
• 负责人: John Werren
• 金额: $ 33万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9981634&HistoricalAwards=false
• 关键词: Genetics; Hybrid; Incompatibility; Nasonia

9981634Werren By its very nature, hybrid lethality involves interactions among genes. Hybrid lethals do not cause lethality in their own genetic background, but do cause lethality in hybrids, due to negative interactions among genes from the different species. For this reason, genetic studies of hybrid lethals can be used to uncover a "web" of genetic interactions. This project will investigate the genetic basis of hybrid lethality between two species of Nasonia insects. This insect is particularly suited for studying complex gene interactions because the males are haploid (have only one set of chromosomes), making the genetic analysis much more simple. Basic objectives are to (a) genetically characterize sets of interacting genes that cause hybrid lethality and (b) fine-scale map hybrid lethals for future cloning. The long-term objectives of this research are to clone and sequence genes involved in hybrid lethality and to determine how these genes interact. Results will yield important information on the nature of genetic interactions that cause lethality. In addition, these studies could yield novel genes and gene interactions not detected by traditional mutational genetic approaches. Because of the surprising conservation of gene function found across diverse organisms (including from insects to humans), gene interactions found in Nasonia could also occur in mammals, including humans.

9981634Werren就其本质而言，杂交致死性涉及基因之间的相互作用。 杂交致死在其自身的遗传背景中不引起致死，但由于来自不同物种的基因之间的负相互作用，在杂交种中引起致死。 出于这个原因，杂交致死的遗传研究可以用来揭示一个“网络”的遗传相互作用。本计画将探讨两种纳苏虫杂交致死的遗传基础。这种昆虫特别适合研究复杂的基因相互作用，因为雄性是单倍体（只有一套染色体），使遗传分析更加简单。 基本目标是（a）从遗传学上鉴定引起杂种致死性的相互作用基因的集合和（B）为将来的克隆绘制精细比例的杂种致死图。这项研究的长期目标是克隆和测序与杂种致死性有关的基因，并确定这些基因如何相互作用。研究结果将产生关于导致致死性的遗传相互作用的性质的重要信息。 此外，这些研究可能产生传统突变遗传学方法无法检测到的新基因和基因相互作用。由于在不同生物体（包括从昆虫到人类）中发现的基因功能惊人的保守性，在Nasonia中发现的基因相互作用也可能发生在包括人类在内的哺乳动物中。