The characterization of multinucleated giant cells in granulomatous inflammation

肉芽肿性炎症中多核巨细胞的特征

• 批准号: 137891782
• 负责人: Dr. Laura Helming
• 金额: --
• 依托单位: Merck KGaA
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/137891782?language=en
• 关键词: characterization; multinucleated; giant; cells; granulomatous

Multinucleated giant cells have long been recognized as a characteristic feature of chronic granulomatous responses occurring in tuberculosis, schistosomiasis, sarcoidosis, the foreign body reaction and other granulomatous diseases. They originate from fusion of macrophages and can be generated in vitro by exposure of macrophages to the cytokine interleukin-4. Even though multinucleated giant cells are a hallmark in granulomatous responses, their functional significance is currently unknown. In order to obtain a better understanding of the function of multinucleated giant cells and their potential contribution to disease outcome, we are using in vivo and in vitro approaches. We are investigating granulomatous responses induced by the parasite Schistosoma mansoni in mice deficient in known components of the macrophage fusion machinery. In addition, we are performing a comprehensive phenotypic and functional analysis of multinucleated giant cells in the context of granulomas as well as in culture using mRNA expression profiling and functional assays.

多核巨细胞长期以来被认为是结核病、血吸虫病、结节病、异物反应和其他肉芽肿性疾病中慢性肉芽肿反应的特征性特征。它们起源于巨噬细胞的融合，并且可以通过将巨噬细胞暴露于细胞因子白细胞介素-4而在体外产生。尽管多核巨细胞是肉芽肿反应的标志，但其功能意义目前尚不清楚。为了更好地了解多核巨细胞的功能及其对疾病结局的潜在影响，我们正在使用体内和体外方法。我们正在研究由曼氏血吸虫在缺乏巨噬细胞融合机制已知成分的小鼠中诱导的肉芽肿反应。此外，我们正在使用mRNA表达谱和功能测定对肉芽肿背景下以及培养中的多核巨细胞进行全面的表型和功能分析。