RUI: Cation-Chloride Cotransporters in Gills of the Fiddler Crab, Uca sp.

RUI：招潮蟹鳃中的阳离子-氯化物协同转运蛋白，Uca sp。

• 批准号: 9982375
• 负责人: Christopher Gillen
• 金额: $ 3万
• 依托单位: Kenyon College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9982375&HistoricalAwards=false
• 关键词: RUI; Cation; Chloride; Cotransporters; Gills

Many crabs are able to survive in environments where salinity fluctuates widely, although this ability varies substantially even within a given genus. Osmoregulation, maintaining a constant internal salinity when external salinity fluctuates, requires transport of salt into or out of the body. Salt transport occurs across specialized cell layers and is mediated by salt transport proteins.This work will focus on development of an antibody against invertebrate salt (Na-K-Cl) cotransporters and will demonstrate the ability to detect Na-K-Cl cotransporters in fiddler crabs. Developing species-specific antibodies against fiddler crab cotransporters will require cloning transporters from each species. The full-length sequence of the blue crab Na-K-Cl cotransporter is now in the sequence database. This sequence from a related organism will facilitate the cloning efforts. Preliminary evidence will be collected to demonstrate that invertebrate cation-chloride cotransporters can be successfully expressed and characterized using the Sf9 cell expression system. Data will be accumulated to show that there are changing expression patterns in gill proteins after salinity stress. In particular, these analyses will focus on demonstrating differences between anterior and posterior gills.

许多螃蟹能够在盐度波动很大的环境中生存，尽管这种能力即使在给定的属内也有很大差异。渗透调节，在外部盐度波动时保持恒定的内部盐度，需要将盐输送进或运出体内。盐的转运发生在特定的细胞层，并由盐转运蛋白介导。这项工作将集中在开发一种针对无脊椎动物盐(Na-K-Cl)共转运蛋白的抗体，并将展示在梭子蟹中检测Na-K-Cl共转运蛋白的能力。开发针对招潮蟹共转运蛋白的物种特异性抗体将需要克隆每个物种的转运蛋白。蓝蟹Na-K-Cl共转运蛋白的全长序列现已在序列数据库中。来自相关生物的这个序列将为克隆工作提供便利。利用Sf9细胞表达系统可以成功表达和鉴定无脊椎动物的阳离子-氯离子共转运蛋白。将积累的数据表明，在盐胁迫后，鳃蛋白的表达模式发生了变化。特别是，这些分析将集中于展示前鳃和后鳃之间的差异。