RUI: Cation-Chloride Cotransporters in Gills of the Fiddler Crab, Uca sp.

许多螃蟹能够在盐度波动很大的环境中生存，尽管这种能力在特定的属内也有很大的差异。 渗透调节，当外部盐度波动时保持恒定的内部盐度，需要将盐运输到体内或体外。 盐的转运是通过盐转运蛋白介导的，它可以跨越特定的细胞层，本工作将致力于开发抗无脊椎动物盐（Na-K-Cl）共转运蛋白的抗体，并将证明其检测招潮蟹中Na-K-Cl共转运蛋白的能力。 开发针对招潮蟹协同转运蛋白的物种特异性抗体将需要从每个物种克隆转运蛋白。 蓝蟹钠-钾-氯共转运蛋白的全长序列现已在序列数据库中。 来自相关生物体的这一序列将有助于克隆工作。 初步的证据将被收集，以证明无脊椎动物的阳离子-氯离子协同转运蛋白可以成功地表达和使用Sf 9细胞表达系统的特点。 本研究将累积资料，以显示盐度胁迫后鳃蛋白质的表达模式有所改变。 特别是，这些分析将集中在证明前鳃和后鳃之间的差异。

Many crabs are able to survive in environments where salinity fluctuates widely, although this ability varies substantially even within a given genus. Osmoregulation, maintaining a constant internal salinity when external salinity fluctuates, requires transport of salt into or out of the body. Salt transport occurs across specialized cell layers and is mediated by salt transport proteins.This work will focus on development of an antibody against invertebrate salt (Na-K-Cl) cotransporters and will demonstrate the ability to detect Na-K-Cl cotransporters in fiddler crabs. Developing species-specific antibodies against fiddler crab cotransporters will require cloning transporters from each species. The full-length sequence of the blue crab Na-K-Cl cotransporter is now in the sequence database. This sequence from a related organism will facilitate the cloning efforts. Preliminary evidence will be collected to demonstrate that invertebrate cation-chloride cotransporters can be successfully expressed and characterized using the Sf9 cell expression system. Data will be accumulated to show that there are changing expression patterns in gill proteins after salinity stress. In particular, these analyses will focus on demonstrating differences between anterior and posterior gills.