CAREER: Characterization, Inhibition, and Reversal of Protein Aggregation

职业：蛋白质聚集的表征、抑制和逆转

• 批准号: 9984312
• 负责人: Anne Robinson
• 金额: $ 52万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9984312&HistoricalAwards=false
• 关键词: CAREER; Characterization; Inhibition; Reversal; Protein

The objective of this project is to study protein folding by examining protein folding intermediates that are critical branching points for aggregation side reactions as well as mutagenic and physical means for disfavoring these side reactions. The specific project aims are to: (1) measure individual rate constants and develop a mechanistic model for formation of tailspike aggregation and assembly intermediates, (2) identify the rate controlling steps and develop strategies to inhibit and reverse tailspike aggregation, and (3) measure secondary structure and supermolecular morphology of aggregates using circular dichroism and atomic force microscopy.

该项目的目的是通过检查蛋白质折叠中间体来研究蛋白质折叠，这是聚集侧反应的关键分支点，以及诱变和物理手段，以不利于这些副反应。 具体项目的目的是：（1）衡量单个速率常数并开发一种机械模型，以形成尾式雪橇和组装中间体，（2）确定速率控制步骤并制定策略，以抑制和反向尾巴凝聚，以及（3）使用圆形结构和使用圆形二十一章的二十一个二核和超级分子形态的方法来抑制和反向尾型式旋转。