CAREER: Characterization, Inhibition, and Reversal of Protein Aggregation

职业：蛋白质聚集的表征、抑制和逆转

• 批准号: 9984312
• 负责人: Anne Robinson
• 金额: $ 52万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9984312&HistoricalAwards=false
• 关键词: CAREER; Characterization; Inhibition; Reversal; Protein

The objective of this project is to study protein folding by examining protein folding intermediates that are critical branching points for aggregation side reactions as well as mutagenic and physical means for disfavoring these side reactions. The specific project aims are to: (1) measure individual rate constants and develop a mechanistic model for formation of tailspike aggregation and assembly intermediates, (2) identify the rate controlling steps and develop strategies to inhibit and reverse tailspike aggregation, and (3) measure secondary structure and supermolecular morphology of aggregates using circular dichroism and atomic force microscopy.

该项目的目的是通过检查蛋白质折叠中间体来研究蛋白质折叠，这些中间体是聚集副反应的关键分支点，以及不利于这些副反应的诱变和物理手段。具体的项目目标是：(1)测量单个速率常数并建立尾穗聚集和组装中间体形成的机制模型；(2)确定速率控制步骤并制定抑制和逆转尾穗聚集的策略；(3)使用圆二色性和原子力显微镜测量聚集体的二级结构和超分子形态。