POWRE: Molecular Determinants and Inhibition of Protein Aggregation

9720570罗宾逊蛋白质的错误折叠或错误组装是生物技术工业、生物化学研究和人类疾病中的主要问题。 折叠问题限制了商业上重要的蛋白质的生产，并且从聚集体中回收天然蛋白质的现有方法通常效率低且耗时。 聚集也已被鉴定为许多人类疾病的原因，包括囊性纤维化、阿尔茨海默病和朊病毒海绵状脑病如克-雅二氏病。 为了解决生产中的聚集问题并了解体内和体外驱动聚集的分子决定因素，研究计划是通过应用静水压力来研究P22尾穗蛋白聚集的抑制和逆转。 所获得的结果将作为研究其他重要的蛋白质系统的基础，无论是工业还是医学相关性。 ***

9720570 Robinson Misfolding or misassembly of proteins is a major problem in the biotechnology industry, in biochemical research, and in human disease. Folding problems limit production of commercially important proteins, and existing methods of recovering native protein from aggregates are often inefficient and time-consuming. Aggregation has also been identified as the cause of a number of human diseases, including cystic fibrosis, Alzheimer's disease, and prion spongiform encephalopathies such as Creutzfeldt-Jacob disease. In order to address the aggregation problems in production and to understand the molecular determinants that drive aggregation in vivo and in vitro, the research plan is to investigate the inhibition and reversal of protein aggregation of P22 tailspike by application of hydrostatic pressure. The results obtained will serve as a foundation for studying other important protein systems, of both industrial and medical relevance. ***