POWRE: Molecular Determinants and Inhibition of Protein Aggregation

POWRE：分子决定因素和蛋白质聚集的抑制

• 批准号: 9720570
• 负责人: Anne Robinson
• 金额: $ 8.5万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9720570&HistoricalAwards=false
• 关键词: POWRE; Molecular; Determinants; Inhibition; Protein

9720570 Robinson Misfolding or misassembly of proteins is a major problem in the biotechnology industry, in biochemical research, and in human disease. Folding problems limit production of commercially important proteins, and existing methods of recovering native protein from aggregates are often inefficient and time-consuming. Aggregation has also been identified as the cause of a number of human diseases, including cystic fibrosis, Alzheimer's disease, and prion spongiform encephalopathies such as Creutzfeldt-Jacob disease. In order to address the aggregation problems in production and to understand the molecular determinants that drive aggregation in vivo and in vitro, the research plan is to investigate the inhibition and reversal of protein aggregation of P22 tailspike by application of hydrostatic pressure. The results obtained will serve as a foundation for studying other important protein systems, of both industrial and medical relevance. ***

9720570罗宾逊蛋白质的错误折叠或错误组装是生物技术行业、生化研究和人类疾病中的一个主要问题。折叠问题限制了具有商业意义的蛋白质的生产，而且现有的从聚集体中回收天然蛋白质的方法往往效率低下且耗时。聚集也被确定为许多人类疾病的原因，包括囊性纤维化、阿尔茨海默病和普恩海绵状脑病，如克雅氏病。为了解决生产中的聚集问题，了解驱动体内和体外聚集的分子决定因素，本研究计划通过施加静水压力来研究P22尾穗蛋白聚集的抑制和逆转。所获得的结果将作为研究其他具有工业和医学意义的重要蛋白质系统的基础。***