POWRE: Molecular Determinants and Inhibition of Protein Aggregation

POWRE：分子决定因素和蛋白质聚集的抑制

• 批准号: 9720570
• 负责人: Anne Robinson
• 金额: $ 8.5万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9720570&HistoricalAwards=false
• 关键词: POWRE; Molecular; Determinants; Inhibition; Protein

9720570 Robinson Misfolding or misassembly of proteins is a major problem in the biotechnology industry, in biochemical research, and in human disease. Folding problems limit production of commercially important proteins, and existing methods of recovering native protein from aggregates are often inefficient and time-consuming. Aggregation has also been identified as the cause of a number of human diseases, including cystic fibrosis, Alzheimer's disease, and prion spongiform encephalopathies such as Creutzfeldt-Jacob disease. In order to address the aggregation problems in production and to understand the molecular determinants that drive aggregation in vivo and in vitro, the research plan is to investigate the inhibition and reversal of protein aggregation of P22 tailspike by application of hydrostatic pressure. The results obtained will serve as a foundation for studying other important protein systems, of both industrial and medical relevance. ***

9720570 Robinson 蛋白质的错误折叠或错误组装是生物技术工业、生化研究和人类疾病中的一个主要问题。 折叠问题限制了商业上重要的蛋白质的生产，并且现有的从聚集物中回收天然蛋白质的方法通常效率低下且耗时。 聚集也被确定为许多人类疾病的原因，包括囊性纤维化、阿尔茨海默氏病和朊病毒海绵状脑病，例如克雅氏病。 为了解决生产中的聚集问题并了解体内和体外驱动聚集的分子决定因素，该研究计划是研究应用静水压抑制和逆转 P22 尾尖蛋白聚集。 获得的结果将作为研究其他具有工业和医学相关性的重要蛋白质系统的基础。 ***