Collaborative Proposal: Exploiting synthetic GPCRs and mating factors as extracellular sensors for substrate-dependent assembly of complex cellulosomes

合作提案:利用合成 GPCR 和交配因子作为细胞外传感器,用于复杂多纤维素酶体的底物依赖性组装

基本信息

  • 批准号:
    1263768
  • 负责人:
  • 金额:
    $ 18万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2013
  • 资助国家:
    美国
  • 起止时间:
    2013-07-01 至 2017-06-30
  • 项目状态:
    已结题

项目摘要

1263719/1263768/1263799Chen/Robinson/Da Silva The primary obstacle impeding the more widespread use of biomass for energy and chemical production is the absence of a low-cost technology for overcoming the recalcitrance of these materials. Cellulosomes are self-assembled multi-enzyme complexes found in many anaerobic microorganisms that are highly efficient for biomass depolymerization. The group of investigators have successfully developed a synthetic yeast consortium displaying a functional cellulosome for biofuel production. However, given the complexity of the different members involved in the consortium, engineering cell to cell coordination is the key in maintaining the required overall functionality. Their goal in this project is to design synthetic extracellular sensors that can be used to regulate the secretion of enzymes and adaptor scaffoldins involved in the assembly of the appropriate complex cellulosome structure in a substrate-dependent manner. The proposed research is scientifically significant because it will provide a general framework for the design of extracellular sensors that are important for other bioprocessing or biomedical applications. Moreover, graduate students participating in this research will gain an integrated perspective of the important interfaces and synergies connecting biochemistry, modern genetics, and process engineering to solve an important problem of our society.
阻碍生物质更广泛地用于能源和化学品生产的主要障碍是缺乏克服这些材料的不稳定性的低成本技术。纤维素体是在许多厌氧微生物中发现的自组装的多酶复合物,其对于生物质解聚是高效的。研究小组已经成功地开发出一种合成酵母财团,展示了用于生物燃料生产的功能性纤维素酶体。然而,考虑到联盟中涉及的不同成员的复杂性,工程小区到小区协调是维持所需的整体功能的关键。他们在这个项目中的目标是设计合成的细胞外传感器,可用于调节酶和接头支架蛋白的分泌,这些酶和接头支架蛋白参与以底物依赖性方式组装适当的复杂纤维素体结构。这项拟议的研究具有科学意义,因为它将为细胞外传感器的设计提供一个通用框架,这些传感器对于其他生物加工或生物医学应用非常重要。此外,参加这项研究的研究生将获得连接生物化学,现代遗传学和工艺工程的重要接口和协同作用的综合视角,以解决我们社会的重要问题。

项目成果

期刊论文数量(0)
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Anne Robinson其他文献

R142: The Effects of Sensory Deprivation on Olfactory Thresholds
  • DOI:
    10.1016/j.otohns.2007.06.478
  • 发表时间:
    2007-08-01
  • 期刊:
  • 影响因子:
  • 作者:
    Julian Anthony Gaskin;Anne Robinson;Carl M. Philpott;Paul C. Goodenough;Allan Clark;George E. Murty
  • 通讯作者:
    George E. Murty
The evolving nature of narcotic use in northwestern Ontario.
安大略省西北部麻醉品使用性质的演变。
  • DOI:
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    1.1
  • 作者:
    Jazmyn Balfour;Sara Rea;Janet N Gordon;J. Dooley;L. Kelly;Anne Robinson
  • 通讯作者:
    Anne Robinson
Sa1208 Sustained Efficacy in Patients With Ulcerative Colitis Treated With Adalimumab: Results From ULTRA 2
  • DOI:
    10.1016/s0016-5085(13)60814-8
  • 发表时间:
    2013-05-01
  • 期刊:
  • 影响因子:
  • 作者:
    Subrata Ghosh;Douglas C. Wolf;William Sandborn;Jean-Frederic Colombel;Qian Zhou;Andreas Lazar;Samantha Eichner;Anne Robinson;Roopal Thakkar
  • 通讯作者:
    Roopal Thakkar
Sa1213 Safety of Adalimumab in Global Clinical Trials of Ulcerative Colitis Patients
  • DOI:
    10.1016/s0016-5085(13)60819-7
  • 发表时间:
    2013-05-01
  • 期刊:
  • 影响因子:
  • 作者:
    Jean-Frederic Colombel;Subrata Ghosh;William Sandborn;Gert A. Van Assche;Walter Reinisch;Andreas Lazar;Samantha Eichner;Bidan Huang;Anne Robinson;Roopal Thakkar
  • 通讯作者:
    Roopal Thakkar
The value of PubMed and HighWire Press for the busy general practitioner
PubMed 和 HighWire Press 对于忙碌的全科医生的价值
  • DOI:
    10.18773/austprescr.2004.010
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    2.7
  • 作者:
    Anne Robinson;S. Day
  • 通讯作者:
    S. Day

Anne Robinson的其他文献

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{{ truncateString('Anne Robinson', 18)}}的其他基金

GOALI: Collaborative Proposal: Mechanistic Design of Aggregation Resistance in Multi-Domain Proteins
GOALI:合作提案:多域蛋白质抗聚集的机制设计
  • 批准号:
    1264554
  • 财政年份:
    2013
  • 资助金额:
    $ 18万
  • 项目类别:
    Standard Grant
Understanding trafficking and membrane localization to re-engineer host and GPCR protein for improved expression
了解运输和膜定位以重新设计宿主和 GPCR 蛋白以改善表达
  • 批准号:
    1249200
  • 财政年份:
    2012
  • 资助金额:
    $ 18万
  • 项目类别:
    Standard Grant
Understanding trafficking and membrane localization to re-engineer host and GPCR protein for improved expression
了解运输和膜定位以重新设计宿主和 GPCR 蛋白以改善表达
  • 批准号:
    1033268
  • 财政年份:
    2010
  • 资助金额:
    $ 18万
  • 项目类别:
    Standard Grant
Biochemical Engineering XVI: Past, Present, and Future. Held in Burlington, VT from July 5-9, 2009
生物化学工程十六:过去、现在和未来。
  • 批准号:
    0936044
  • 财政年份:
    2009
  • 资助金额:
    $ 18万
  • 项目类别:
    Standard Grant
IGERT: Multidisciplinary Graduate Progrqam in Biotechnology
IGERT:生物技术多学科研究生课程
  • 批准号:
    0221651
  • 财政年份:
    2002
  • 资助金额:
    $ 18万
  • 项目类别:
    Continuing Grant
CAREER: Characterization, Inhibition, and Reversal of Protein Aggregation
职业:蛋白质聚集的表征、抑制和逆转
  • 批准号:
    9984312
  • 财政年份:
    2000
  • 资助金额:
    $ 18万
  • 项目类别:
    Continuing Grant
POWRE: Molecular Determinants and Inhibition of Protein Aggregation
POWRE:分子决定因素和蛋白质聚集的抑制
  • 批准号:
    9720570
  • 财政年份:
    1997
  • 资助金额:
    $ 18万
  • 项目类别:
    Standard Grant

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