Collaborative Proposal: Exploiting synthetic GPCRs and mating factors as extracellular sensors for substrate-dependent assembly of complex cellulosomes

合作提案：利用合成 GPCR 和交配因子作为细胞外传感器，用于复杂多纤维素酶体的底物依赖性组装

• 批准号: 1263768
• 负责人: Anne Robinson
• 金额: $ 18万
• 依托单位: Tulane University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1263768&HistoricalAwards=false
• 关键词: Collaborative; Proposal; Exploiting; synthetic; GPCRs

1263719/1263768/1263799Chen/Robinson/Da Silva The primary obstacle impeding the more widespread use of biomass for energy and chemical production is the absence of a low-cost technology for overcoming the recalcitrance of these materials. Cellulosomes are self-assembled multi-enzyme complexes found in many anaerobic microorganisms that are highly efficient for biomass depolymerization. The group of investigators have successfully developed a synthetic yeast consortium displaying a functional cellulosome for biofuel production. However, given the complexity of the different members involved in the consortium, engineering cell to cell coordination is the key in maintaining the required overall functionality. Their goal in this project is to design synthetic extracellular sensors that can be used to regulate the secretion of enzymes and adaptor scaffoldins involved in the assembly of the appropriate complex cellulosome structure in a substrate-dependent manner. The proposed research is scientifically significant because it will provide a general framework for the design of extracellular sensors that are important for other bioprocessing or biomedical applications. Moreover, graduate students participating in this research will gain an integrated perspective of the important interfaces and synergies connecting biochemistry, modern genetics, and process engineering to solve an important problem of our society.

1263719/1263768/1263799CHEN/ROBINSON/DA SILVA的主要障碍阻碍了更广泛地将生物质用于能源和化学生产的主要障碍，这是缺乏克服这些材料重降低这些材料的低成本技术。纤维素是在许多厌氧微生物中发现的自组装的多酶复合物，它们对生物质解聚高效。研究人员已经成功地开发了一个合成酵母菌财团，该联盟显示了用于生物燃料生产的功能性纤维素。但是，鉴于涉及财团的不同成员的复杂性，工程细胞与细胞配位是维持所需的总体功能的关键。他们在这个项目中的目标是设计合成的细胞外传感器，可用于调节与底物相关方式组装参与适当复杂纤维体结构的酶和衔接子支架的分泌。拟议的研究在科学上是重要的，因为它将为设计对其他生物处理或生物医学应用很重要的细胞外传感器的设计提供一般框架。此外，参加这项研究的研究生将获得连接生物化学，现代遗传学和过程工程的重要界面和协同作用的综合视角，以解决我们社会的重要问题。