Electrical and Optical Monitoring of Facilitation Mechanisms in Chromaffin Cells

[85559 .]张建军，张建军，张建军，等。内分泌和神经系统的某些细胞在受到电刺激时释放化学物质，如激素和神经递质。这些释放的化学物质到达目标细胞或组织，并产生反应。激素和神经递质的调节释放的知识对于我们理解内分泌、神经和心血管系统的正常和异常功能至关重要，但仍然有限。该提案概述了一系列实验，旨在提供对释放过程及其监管的更详细描述。这项研究的目的是阐明细胞控制激素或神经递质释放量的机制，以及细胞如何为随后的活动做好准备。电学、光学和计算技术将用于测量刺激诱发的激素和神经递质的释放，以及量化和描述释放过程中的单个步骤。从更广泛的意义上说，了解生物系统如何运输和传递分子到细胞隔间可能有助于未来设计靶向递送生物活性化合物的纳米技术。在佐治亚医学院扩大这一研究方案将有助于在这些最先进的技术方面对科学人员进行培训。最后，这项研究的资金投入将加强生物技术进一步发展所需的科学基础设施

985559smith, c.Electrical and Optical Monitoring of Facilitation Mechanisms in Chromaffin Cells. Certain cells of the endocrine and nervous system release chemical substances such as hormones and neurotransmitter in response to electrical stimulation. These released chemical substances travel to target cells or tissues and effect a response. Knowledge of the regulated release of hormones and neurotransmitters is critical to our understanding of the normal, and abnormal, function of the endocrine, nervous and cardiovascular systems, yet remains limited. This proposal outlines a series of experiments designed to furnish a more detailed description of the release process, and its regulation. It is the goal of this research to elucidate mechanisms by which cells control the amount of hormone or neurotransmitter released, and how the cells then ready themselves for subsequent activity. Electrical, optical and computational techniques will be employed to measure the stimulation-evoked release of hormone and neurotransmitter from single cells, as well as to quantify and describe the single steps along the release process. In a broader sense, understanding how biological systems transport and deliver molecules to cellular compartments may aid in the future design of nanotechnologies for the targeted delivery of bio-active compounds. The expansion of this research program at the Medical College of Georgia will facilitate training of scientific personnel in these state-of -the-art technologies. Finally, the capital investment in this research will strengthen the scientific infrastructure required for the further development of biotechnologies