Electrical and Optical Monitoring of Facilitation Mechanisms in Chromaffin Cells

嗜铬细胞中促进机制的电学和光学监测

• 批准号: 9985559
• 负责人: Corey Smith
• 金额: $ 42.11万
• 依托单位: AUGUSTA UNIVERSITY RESEARCH INSTITUTE, INC.
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9985559&HistoricalAwards=false
• 关键词: Electrical; Optical; Monitoring; Facilitation; Mechanisms

985559smith, c.Electrical and Optical Monitoring of Facilitation Mechanisms in Chromaffin Cells. Certain cells of the endocrine and nervous system release chemical substances such as hormones and neurotransmitter in response to electrical stimulation. These released chemical substances travel to target cells or tissues and effect a response. Knowledge of the regulated release of hormones and neurotransmitters is critical to our understanding of the normal, and abnormal, function of the endocrine, nervous and cardiovascular systems, yet remains limited. This proposal outlines a series of experiments designed to furnish a more detailed description of the release process, and its regulation. It is the goal of this research to elucidate mechanisms by which cells control the amount of hormone or neurotransmitter released, and how the cells then ready themselves for subsequent activity. Electrical, optical and computational techniques will be employed to measure the stimulation-evoked release of hormone and neurotransmitter from single cells, as well as to quantify and describe the single steps along the release process. In a broader sense, understanding how biological systems transport and deliver molecules to cellular compartments may aid in the future design of nanotechnologies for the targeted delivery of bio-active compounds. The expansion of this research program at the Medical College of Georgia will facilitate training of scientific personnel in these state-of -the-art technologies. Finally, the capital investment in this research will strengthen the scientific infrastructure required for the further development of biotechnologies

985559smith，c.嗜铬细胞中易化机制的电学和光学监测。内分泌和神经系统的某些细胞释放化学物质，如激素和神经递质，以响应电刺激。这些释放的化学物质到达靶细胞或组织并产生反应。激素和神经递质的调节释放的知识对于我们理解内分泌、神经和心血管系统的正常和异常功能至关重要，但仍然有限。该提案概述了一系列旨在更详细地描述释放过程及其调节的实验。这项研究的目的是阐明细胞控制激素或神经递质释放量的机制，以及细胞如何为随后的活动做好准备。电，光和计算技术将被用来测量刺激诱发的激素和神经递质从单个细胞的释放，以及量化和描述的单个步骤沿着释放过程。从更广泛的意义上说，了解生物系统如何运输和提供分子到细胞区室可能有助于未来设计的纳米技术，有针对性地提供生物活性化合物。这项研究计划在格鲁吉亚医学院的扩展将促进科学人员在这些国家的最先进的技术的培训。最后，对这项研究的资本投资将加强生物技术进一步发展所需的科学基础设施