SGER: Crystallization of a P1-Type Heavy Metal Transport ATPase

SGER：P1 型重金属转运 ATP 酶的结晶

• 批准号: 9985771
• 负责人: Jose Arguello
• 金额: $ 9.96万
• 依托单位: Worcester Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9985771&HistoricalAwards=false
• 关键词: SGER; Crystallization; P1; Type; Heavy

9985771ArguelloIons are transported across membranes of living organisms using metabolic energy derived from ATP. The transport proteins are enzymes that are imbedded in the membrane and classified as P-type ATPases. There is not a complete understanding of the processes performed by these enzymes due in part to a lack of knowledge of their atomic structures. During the last few years, a new group of P-type ATPases (P1-type) has been identified in prokaryotes that transport heavy metals such as Cu+1, Cu+2, Zn+2, Cd+2, and others into cells. These enzymes have the same mechanistic characteristics as other P-type ATPases, but they are smaller in size. This characteristic makes them easier to isolate, purify, crystallize and obtain their atomic structure. The detailed structural information obtained through crystallographic analysis of this sub-class of P-type ATPases will provide information on the ion selectivity and energy transduction mechanisms of other P-type ATPases and a clearer understanding of energy coupled ion transport. This is especially important since this knowledge may also provide a basis for the engineering of microorganisms that can be used effectively in bioremediation.

9985771利用ATP产生的代谢能，Arguelloon离子可穿过生物体的细胞膜。 转运蛋白是包埋在膜中的酶，被分类为P型ATP酶。 由于缺乏对这些酶的原子结构的了解，对这些酶进行的过程没有完全的了解。 在过去的几年中，一组新的P型ATP酶（P1型）已被确定在原核生物中，运输重金属，如Cu+1，Cu+2，Zn+2，Cd+2，和其他进入细胞。这些酶具有与其他P-型ATP酶相同的机制特征，但它们的尺寸较小。 这种特性使它们更容易分离、纯化、结晶并获得其原子结构。 通过对P型ATP酶这一亚类的晶体学分析获得的详细结构信息将提供有关其他P型ATP酶的离子选择性和能量转换机制的信息，并更清楚地了解能量耦合离子传输。 这是特别重要的，因为这方面的知识也可以提供一个基础，为工程的微生物，可以有效地用于生物修复。