Highly Preorganized Artificial Phosphoesterases
高度预组织的人工磷酸酯酶
基本信息
- 批准号:9988615
- 负责人:
- 金额:$ 34.1万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-08-01 至 2004-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
With this award the Organic and Macromolecular Chemistry Program continues its support for the work of Dr. Eric V. Anslyn in the Department of Chemistry at the University of Texas at Austin. The work aims to prepare artificial phosphoesterase enzymes capable of cleaving RNA with rates and selectivities rivaling those of the natural enzymes. The approach involves systematic structural variations around a rigid hexasubstituted benzene core with attached electrophilic Zn(+2) and guanidinium cation sites. Cooperativity between metal ion sites and between metal ions and guanidinium will be investigated using kinetics, measurements of equilibrium binding constants, pH titrations, and crystallography.Artificial enzymes which can cleave DNA will be developed by making small tight cavities in which DNA can form at least four and as many as six hydrogen bonds to positively charged zinc or guanidinium ions. The keys to these new catalysts are the large number of potential binding interactions and the rigidity of the cavity walls. If effective catalysts can be developed they would be useful in gene therapy. The PI has a record of being an excellent teacher and research mentor, and has helped a number of female graduate students to go on to get PhDs.
凭借这一奖项,有机和高分子化学项目继续支持德克萨斯大学奥斯汀分校化学系埃里克·V·安斯林博士的工作。这项工作旨在制备能够以与天然酶相媲美的速率和选择性来裂解RNA的人工磷酸酯酶。该方法涉及到刚性的六取代苯核周围的系统结构变化,以及附着的亲电锌(+2)和胍阳离子中心。将通过动力学、平衡结合常数的测量、pH滴定和结晶学来研究金属离子中心之间以及金属离子和胍离子之间的协作性。可以通过制造小而紧密的空腔来开发能够切割DNA的人造酶,在空腔中DNA可以与带正电的锌离子或胍离子形成至少四个甚至多达六个氢键。这些新型催化剂的关键是大量潜在的结合相互作用和腔壁的刚性。如果能够开发出有效的催化剂,它们将在基因治疗中发挥作用。PI作为一名优秀的教师和研究导师的记录,并帮助了一些女研究生继续获得博士学位。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Eric Anslyn其他文献
Eric Anslyn的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Eric Anslyn', 18)}}的其他基金
Emergence of Structure and Function from Sequenceable Sequence-Defined Macrocyclic Oligourethanes
可测序序列定义的大环低聚聚氨酯的结构和功能的出现
- 批准号:
2203354 - 财政年份:2022
- 资助金额:
$ 34.1万 - 项目类别:
Standard Grant
GOALI: Utilizing Rapid Assays for Determining Enantiomeric Excess and Catalyst Discovery in Pharma
GOALI:利用快速检测确定制药中的对映体过量和催化剂发现
- 批准号:
1665040 - 财政年份:2017
- 资助金额:
$ 34.1万 - 项目类别:
Continuing Grant
Mechanistic and Catalytic Studies of Reversible Covalent Bonding
可逆共价键的机理和催化研究
- 批准号:
1212971 - 财政年份:2012
- 资助金额:
$ 34.1万 - 项目类别:
Standard Grant
Optical Methods for EE Analysis of Simple Carboxylic Acids
简单羧酸的 EE 分析光学方法
- 批准号:
0616467 - 财政年份:2006
- 资助金额:
$ 34.1万 - 项目类别:
Continuing Grant
Carbohydrate Receptors and Sensors
碳水化合物受体和传感器
- 批准号:
9307282 - 财政年份:1993
- 资助金额:
$ 34.1万 - 项目类别:
Continuing Grant
Presidential Young Investigator Award/Development of Artificial Enzymes.
总统青年研究员奖/人工酶的开发。
- 批准号:
9057208 - 财政年份:1990
- 资助金额:
$ 34.1万 - 项目类别:
Continuing Grant
Development of Carbohydrate Complexing Agents
碳水化合物络合剂的开发
- 批准号:
8915872 - 财政年份:1989
- 资助金额:
$ 34.1万 - 项目类别:
Standard Grant
Postdoctoral Research Fellowship in Chemistry
化学博士后研究奖学金
- 批准号:
8808370 - 财政年份:1988
- 资助金额:
$ 34.1万 - 项目类别:
Continuing Grant
相似海外基金
Conformationally Preorganized Diamide Ligands for Enhanced Binding of f-Block Elements: New Ligands, Coordination Complexes and Functional Materials
用于增强 f 区元素结合的构象预组织二酰胺配体:新配体、配位配合物和功能材料
- 批准号:
0213563 - 财政年份:2002
- 资助金额:
$ 34.1万 - 项目类别:
Continuing Grant
Preorganized bi- and trimetallic complexes as building blocks for the controlled assembly of extended coordination compounds with magnetic ordering
预组织双金属和三金属配合物作为构建块,用于通过磁有序控制扩展配位化合物的组装
- 批准号:
5368638 - 财政年份:2002
- 资助金额:
$ 34.1万 - 项目类别:
Priority Programmes