Directed Evolution of A Single-Chain Catalytic Antibody With Chorismate Mustase Activity to Increase Its Ability and Catalytic Efficiency, Using RNA-Protein Fusions

利用 RNA-蛋白质融合定向进化具有分支酸必须酶活性的单链催化抗体，以提高其能力和催化效率

• 批准号: 0000473
• 负责人: Joel Goldberg
• 金额: $ 4.72万
• 依托单位: Goldberg, Joel
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-15 至 2001-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0000473&HistoricalAwards=false
• 关键词: Directed; Evolution; Single; Chain; Catalytic

0000473GoldbergThe International Research Fellow Awards Program enables U.S. scientists and engineers to conduct three to twentyfour months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad.This award will provide Dr.Joel Goldberg with support for 12 months to work with Professor Donald Hilvert at the Swiss Federal Institute of Technology (ETH) in Zurich, Switzerland on the directed molecular evolution of a single-chain catalytic antibody with increased stability and catalytic efficiency. This project is co-funded by NSF's Directorate for Mathematical and Physical Sciences' Office of Multidisciplinary Activities.This project will prepare a library of RNS-protein fusions for the directed evolution of proteins in order to generate mutant antibodies with increased stabilities and altered catalytic properties. The PI and host will start with a catalytic antibody which efficiently converts chorismate to prephenate. This project will provide insights into the mechanisms used by proteins to maintain active, folded conformations and promote chemical reactions.Professor Hilvert is an expert on protein and catalytic antibodies. The entire infrastructure for the Laboratorium fur Organische Chemie and the Department of Chemistry at the ETH are available for this project.

0000473戈德堡国际研究员奖励计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。 该计划的奖项提供了联合研究的机会，以及使用国外独特或互补的设施，专业知识和实验条件。该奖项将为Joel Goldberg博士提供12个月的支持，与位于苏黎世的瑞士联邦理工学院（ETH）的Donald Hilvert教授一起工作，瑞士关于单链催化抗体的定向分子进化，具有增加的稳定性和催化效率。 该项目由美国国家科学基金会数学和物理科学理事会多学科活动办公室共同资助，该项目将为蛋白质的定向进化准备一个RNS-蛋白质融合库，以产生具有增加的稳定性和改变的催化特性的突变抗体。 PI和宿主将从催化抗体开始，该催化抗体有效地将分支酸盐转化为预苯酸盐。 该项目将深入了解蛋白质用于保持活性，折叠构象和促进化学反应的机制。Hilvert教授是蛋白质和催化抗体方面的专家。 ETH有机化学实验室和化学系的整个基础设施可用于该项目。