High-throughput single-molecule directed evolution

DNA聚合酶是许多生物技术工具中必不可少的酶，包括DNA测序和PCR测试。然而，现有的DNA聚合酶有局限性，导致不准确和低效率。现有的改进聚合酶的方法在筛选细微但关键的性状方面缺乏敏感性。该项目旨在通过开发一种新的单分子定向进化系统来进化更好的聚合酶，从而克服这一限制。通过这项新技术，我们的目标是鉴定具有改进性能的DNA聚合酶，从而有利于生物技术应用。此外，这些单分子定向进化方法将使更广泛的科学界受益，并为定向进化的进一步发展奠定基础。

DNA polymerases are essential enzymes in many biotechnological tools, including DNA sequencing and PCR tests. However, existing DNA polymerases have limitations, resulting in inaccuracies and inefficiencies. Existing methods to improve polymerases lack sensitivity to screen for subtle, yet pivotal traits. This project aims to overcome this limitation by developing a new single-molecule directed-evolution system to evolve better polymerases. With this new technology we aim to identify DNA polymerases with improved performance that benefit biotechnological applications. Additionally, these single-molecule directed-evolution methods will benefit the wider scientific community and lay the foundation for further advances in directed evolution.