High-throughput single-molecule directed evolution

高通量单分子定向进化

• 批准号: DP240101399
• 负责人: Dr Lisanne Spenkelink
• 金额: $ 41.37万
• 依托单位: University of Wollongong
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2024
• 资助国家: 澳大利亚
• 起止时间: 2024-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP240101399
• 关键词: throughput; single; molecule; directed; evolution

DNA polymerases are essential enzymes in many biotechnological tools, including DNA sequencing and PCR tests. However, existing DNA polymerases have limitations, resulting in inaccuracies and inefficiencies. Existing methods to improve polymerases lack sensitivity to screen for subtle, yet pivotal traits. This project aims to overcome this limitation by developing a new single-molecule directed-evolution system to evolve better polymerases. With this new technology we aim to identify DNA polymerases with improved performance that benefit biotechnological applications. Additionally, these single-molecule directed-evolution methods will benefit the wider scientific community and lay the foundation for further advances in directed evolution.

DNA聚合酶是许多生物技术工具中必不可少的酶，包括DNA测序和PCR测试。然而，现有的DNA聚合酶具有局限性，导致不准确和效率低下。现有的改进聚合酶的方法缺乏筛选微妙但关键特征的灵敏度。该项目旨在通过开发一种新的单分子定向进化系统来克服这一限制，以进化出更好的聚合酶。通过这项新技术，我们的目标是识别具有改进性能的DNA聚合酶，从而使生物技术应用受益。此外，这些单分子定向进化方法将使更广泛的科学界受益，并为定向进化的进一步发展奠定基础。