High-throughput single-molecule directed evolution

DNA聚合酶是许多生物技术工具中必不可少的酶，包括DNA测序和聚合酶链式反应测试。然而，现有的DNA聚合酶存在局限性，导致不准确和效率低下。现有的改进聚合酶的方法缺乏对筛选细微但关键的特征的敏感性。该项目旨在通过开发一种新的单分子定向进化系统来克服这一限制，以进化出更好的聚合酶。有了这项新技术，我们的目标是鉴定出性能更好、有利于生物技术应用的DNA聚合酶。此外，这些单分子定向进化方法将使更广泛的科学界受益，并为定向进化的进一步进展奠定基础。

DNA polymerases are essential enzymes in many biotechnological tools, including DNA sequencing and PCR tests. However, existing DNA polymerases have limitations, resulting in inaccuracies and inefficiencies. Existing methods to improve polymerases lack sensitivity to screen for subtle, yet pivotal traits. This project aims to overcome this limitation by developing a new single-molecule directed-evolution system to evolve better polymerases. With this new technology we aim to identify DNA polymerases with improved performance that benefit biotechnological applications. Additionally, these single-molecule directed-evolution methods will benefit the wider scientific community and lay the foundation for further advances in directed evolution.