U.S.-Australia Cooperative Research: Use of Genetically Engineered Microbial Enzymes in Calcite Precipitation

美国-澳大利亚合作研究：基因工程微生物酶在方解石沉淀中的应用

• 批准号: 0002608
• 负责人: Sookie Bang
• 金额: $ 14.24万
• 依托单位: South Dakota School of Mines and Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-10-15 至 2004-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0002608&HistoricalAwards=false
• 关键词: U.S.; Australia; Cooperative; Research; Use

0002608BangThe proposal project deals with investigation of the fundamentals of microbiological calcite precipitation at the molecular basis. It will involve a close collaboration between Dr. S.S. Bang, Biochemical Microbiologist, and Dr. V. Ramakrishnan, Civil Engineer, at the South Dakota School of Mines and Technology, Rapid City, SD and Dr. J.R. Warmington, Molecular Microbiologist, and Dr. B.V. Rangan, Civil Engineer, at the Curtin University of Technology, Perth, Australia.The research objectives include 1) biochemical and molecular investigation of urease in calcite precipitation utilizing genetically engineered microorganisms (GEMs), 2) use of immobilized urease and whole cells including GEMs in calcite precipitation, and 3) application of bioremediation technology in in-base and sub-base stabilization as well as remediation of cracks and fissures in various structural formations. Experimental steps will be carried in a coordinated manner to compensate the weaknesses and to complement the strength of each campus.The SDSM&T team has expertise in microbial mineralization and biochemistry, whereas the Australian team has expertise in molecular microbiology. This proposed international collaboration would enable the Australia and the USA. The combined project will result in joining of the complementary skills of the SDSM&T and Australian research teams, exchanging students not only to encourage their participation in the research and but also to train their laboratory research techniques. Combination of these complementary areas of expertise will promote further development and application of the microbial mineralization technology to the future benefits of both countries.

0002608 bang该提案项目涉及微生物方解石沉淀的分子基础基础的研究。它将涉及S.S. Bang博士，生化微生物学家，和V. Ramakrishnan博士，土木工程师，南达科他州矿业与技术学院，拉比德城，SD和J.R.沃明顿博士，分子微生物学家，和B.V. Rangan博士，土木工程师，科廷科技大学，珀斯，澳大利亚密切合作。研究目标包括：1)利用基因工程微生物（GEMs）对方解石沉淀中脲酶的生化和分子研究；2)固定化脲酶和包括GEMs在内的全细胞在方解石沉淀中的应用；3)生物修复技术在碱内和亚碱稳定以及各种结构层裂缝和裂缝修复中的应用。实验步骤将以协调的方式进行，以弥补不足，并补充每个校区的优势。SDSM&amp；T团队在微生物矿化和生物化学方面具有专长，而澳大利亚团队在分子微生物学方面具有专长。这一提议的国际合作将使澳大利亚和美国能够。合并后的项目将结合SDSM&amp；T和澳大利亚研究团队的互补技能，交换学生不仅鼓励他们参与研究，而且还训练他们的实验室研究技术。这些互补的专业领域的结合将促进微生物矿化技术的进一步发展和应用，为两国的未来带来好处。