Construction of nanosyringe arrays for inserting bimolecular motor-powered nanodevices in living cells

用于在活细胞中插入双分子电机驱动的纳米器件的纳米注射器阵列的构建

• 批准号: 0004499
• 负责人: Carlo Montemagno
• 金额: $ 10万
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0004499&HistoricalAwards=false
• 关键词: Construction; nanosyringe; arrays; inserting; bimolecular

AbstractCTS-0004499C. Montemagno, Cornell UniversityAs current technology permits the production of nanostructures smaller than typical cell dimensions, it has become possible to consider useful applications based on their non-destructive, orderly injection inside the cells. In this fashion, cells could be repaired or functionally modified. Of special interest would be the possibility to inject biomolecular motors which could energize the self-assembly of nanostructures inside living cells. This could be accomplished by developing arrays of nanosyringes, each of which to deliver chosen structures on preplanned location and time.The proposed effort will unfold in three steps:1. The fabrication of individual ("standalone") syringes with dimensions suitable for the injection of biomolecular rotary motors (e.g. flagella/ATP), capable of initiating a variety of modifications inside a cell. Silicon wafers will be shaped by existing etching processes, yielding a 10nm diameter for the tips and a 1-micron diameter for the shaft. Testing will be done with green fluorescence protein measurements.2. Since many useful cell modifications may involve a number of different injections, it is desirable to produce, as a first step, a set of self-aligned syringe arrays. These will allow, given a line-up of cells, for simultaneous (and possibly different) injections, followed by other injection patterns if so desired. Testing will be similar to the standalone case.3. The syringes concepts discussed above will be refined: a suspension mechanism will make possible both vertical and horizontal controlled displacements of the syringe. The motion will be tracked with the help of an optical microscope.

摘要CTS-0004499 C。Montemagno，康奈尔大学由于目前的技术允许生产比典型细胞尺寸更小的纳米结构，因此可以考虑基于它们在细胞内的非破坏性，有序注射的有用应用。 以这种方式，细胞可以被修复或功能修饰。 特别令人感兴趣的是注入生物分子马达的可能性，这种马达可以为活细胞内纳米结构的自组装提供能量。 这可以通过开发纳米注射器阵列来实现，每一个纳米注射器都可以在预先计划的位置和时间提供选定的结构。制造具有适合于生物分子旋转马达（例如鞭毛/ATP）的注射的尺寸的单独（“独立”）注射器，其能够在细胞内引发各种修饰。 硅晶片将通过现有的蚀刻工艺成形，尖端直径为10纳米，轴直径为1微米。 将使用绿色荧光蛋白测量进行检测。2.由于许多有用的细胞修饰可能涉及许多不同的注射，因此作为第一步，期望产生一组自对准注射器阵列。 在给定细胞排列的情况下，这些将允许同时（并且可能不同）注射，然后根据需要进行其他注射模式。 测试将类似于独立的情况。3.将改进上面讨论的注射器概念：悬挂机构将使注射器的垂直和水平受控位移成为可能。 这种运动将在光学显微镜的帮助下进行跟踪。