RUI: Acquisition of a Capillary Electrophoresis System for DNA Analysis in Investigations of Adaptation, Speciation, Species Interactions and DNA Repair

RUI：购置毛细管电泳系统，用于适应、物种形成、物种相互作用和 DNA 修复研究中的 DNA 分析

• 批准号: 0070310
• 负责人: Kathryn Jacobson
• 金额: $ 4.71万
• 依托单位: Grinnell College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070310&HistoricalAwards=false
• 关键词: RUI; Acquisition; Capillary; Electrophoresis; System

Abstract JacobsonAutomated capillary electrophoresis system for the analysis of DNA demands relatively little training to use and involves minimum risk of exposure to radioactive or toxic substances. The automated DNA sequencing and fragment sizing (for analysis of DNA polymorphisms) capabilities of the requested instrument will make investigations more efficient, less expensive and more effective learning tools for students. This is central to the institutional goal of integrating research and education of undergraduatesFour of the eight faculty in the Biology Department will use the instrument on a regular basis to explore research questions that span the breadth of the discipline. The immediate goals for instrument use are:- to explore questions relating to the nature of speciation; specifically to develop mitochondrial DNA markers suitable for phylogeographic and population analyses of host races and subspecies of the goldenrod ball-gallmaker; and to generate a DNA sequence-based phylogeny of all extant taxa of Hawaiian tephritid flies.- to examine the causes of correlated evolution in plant breeding systems, life history and morphology; specifically to use DNA polymorphisms to resolve relationships among populations of Clarkia xantiana, and search for markers that correlate with quantitative trait expression.- to further elucidate the molecular basis of DNA repair in response to DNA damage in Acinetobacter; specifically to sequence critical genes involved in this process.- to explore questions relating to community structure of subterranean fungi; specifically to use DNA polymorphisms to quantify genetic variation within and between global populations of desert macrofungi; to continue an ongoing study of the spatial and temporal distribution of variation amongst morel populations; and to explore the spatial distribution of ectomycorrhizal fungal communities associated with bur oak.Genetic analyses will be used to answer questions that reflect the respective disciplines of the investigators, namely microbiology, organismal biology, ecology and evolution. An important goal of the anticipated research will be to provide students with authentic experiences that allow them to understand both principles and applications of DNA techniques. The automated sequencing and sizing capabilities of the requested instrument will also facilitate inclusion of exercises involving DNA sequencing and multilocus fingerprinting in upper-level courses currently being developed as part of a new NSF CCLI-funded biology curriculum at Grinnell College.

摘要雅各布森用于DNA分析的自动化毛细管电泳系统需要相对较少的使用培训，并且暴露于放射性或有毒物质的风险最小。所要求的仪器的自动DNA测序和片段大小（用于分析DNA多态性）能力将使调查更有效，更便宜和更有效的学习工具，为学生。这是中央整合研究和本科生教育的机构目标在生物系的八个教师中的四个将定期使用该仪器来探索跨越学科的广度的研究问题。仪器使用的直接目标是：-探索与物种形成的性质有关的问题;特别是开发适合于对一枝黄花球-gallmaker的宿主种族和亚种进行地理和种群分析的线粒体DNA标记;并生成夏威夷实蝇所有现存分类群的基于DNA序列的系统发育。研究植物育种系统，生活史和形态学相关进化的原因;特别是使用DNA多态性来解决Clarkia xantiana种群之间的关系，并寻找与数量性状表达相关的标记。进一步阐明DNA修复的分子基础，以应对不动杆菌中的DNA损伤;特别是对参与这一过程的关键基因进行测序。探索与地下真菌群落结构有关的问题;特别是利用DNA多态性来量化沙漠大型真菌全球种群内部和之间的遗传变异;继续进行羊肚菌种群之间变异的时空分布研究;并探索与栎属相关的外生菌根真菌群落的空间分布。遗传分析将用于回答以下问题，反映了研究人员各自的学科，即微生物学，有机体生物学，生态学和进化。预期研究的一个重要目标是为学生提供真实的体验，使他们能够理解DNA技术的原理和应用。所要求的仪器的自动测序和尺寸测量能力也将有助于将涉及DNA测序和多位点指纹识别的练习纳入目前正在开发的高级课程，作为格林内尔学院新的NSF CCLI资助的生物课程的一部分。