Acquisition of a ProteinSimple capillary electrophoresis protein immunoanalyzer

购置 ProteinSimple 毛细管电泳蛋白质免疫分析仪

• 批准号: 8949379
• 负责人: Brian C. Kraemer
• 金额: --
• 依托单位: VA PUGET SOUND HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2015-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8949379
• 关键词: Acrylamides; Address; Adoption; Amyotrophic Lateral Sclerosis; Antibodies; Area; Biological Assay; Blood capillaries; Caliber; Capillary Electrophoresis; Cataloging; Catalogs; Computer software; Computers; Consumption; Core Facility; Cost Effectiveness Analysis; DNA Sequence; Data; Data Analyses; Dementia; Detection; Diabetes Mellitus; Effectiveness; Electrophoresis; Enzyme-Linked Immunosorbent Assay; Fees; Hand; Hour; Housing; Human Genome Project; Immobilization; Immunoblot Analysis; Immunoblotting; Individual; Institution; Investigation; Laboratories; Legal patent; Length; Licensing; Manuals; Materials Testing; Methodology; Methods; Minor; Molecular; Molecular Genetics; Molecular Weight; Monitor; Process; Protein Analysis; Proteins; Reagent; Reproducibility; Research; Research Personnel; Research Priority; Resources; Sampling; Services; Signal Transduction; Sodium Dodecyl Sulfate; Standardization; System; Technology; Time; Training; Translational Regulation; Traumatic Brain Injury; Western Blotting; Work; assay development; base; capillary; computerized data processing; cooking; cost; design; instrument; meetings; nanoimmunoassay; nanolitre; preference; programs; public health relevance; research study

 DESCRIPTION (provided by applicant): Request for a ProteinSimple Instrument. We are requesting the purchase of a ProteinSimple capillary electrophoresis protein immunoanalysis system (aka SallySue) for the high throughput cost effective analysis of proteins. This instrument utilizes capillary electrophoretic technology o separate proteins by size like standard sodium dodecyl sulfate poly acrylamide electrophoresis methodology. Subsequent immunodetection functions much like standard chemiluminescent immunoblotting approaches. The main advantages of the SallySue instrument over manual approaches are dramatically reduced hands on time, increased throughput, reduced materials cost, reduced sample consumption, increased quantitative accuracy, increased reproducibility, and lower variability. Adoption of capillary electrophoresis technology for DNA sequencing was the technological breakthrough permitting completion of the human genome project. Application of capillary electrophoresis to protein characterization promises to revolutionize conventional immunoblotting methods. This instrument will be placed in the Molecular Genetics Core facility located in Building 1, Room 710 at VAPSHCS, Seattle; as such it will be available for use by all investigators as a fee for use service. The instrument itself will be operated by trained core staf. Major users of the instrument include Steven Kahn, David Cook, Chang-en Yu, Nicole Liachko, and Brian Kraemer; minor users will be William Banks and Rebecca Hull. Overall, the instrument has utility for any molecular investigation focused on protein and has the potential to replace manual western blotting and ELISA analysis. Overall purchase of this instrument will be expected to increase the competitiveness of VA PSHCS as a research institution and further strengthen the effectiveness and efficiency of its investigators molecular work with protein. The instrument will return its cost many times over in terms of saved time and labor by reducing costs for immunoblotting analysis by up to 60%. Thus research teams will have freed up resources to pursue their objectives more rapidly and in greater depth allowing more rapid progress in research into traumatic brain injury, diabetes, dementia and amyotrophic lateral sclerosis.

 描述（由申请人提供）： 申请ProteinSimple仪器。我们正在寻求购买一个简单的蛋白质毛细管电泳蛋白质免疫分析系统（又名萨利苏）的高通量成本效益的蛋白质分析。该仪器采用毛细管电泳技术，通过大小分离蛋白质，类似于标准十二烷基硫酸钠聚丙烯酰胺电泳方法。随后的免疫检测功能非常类似于标准荧光免疫印迹方法。SallySue仪器相对于手动方法的主要优点是大大减少了手动操作时间、提高了通量、降低了材料成本、减少了样品消耗、提高了定量准确性、提高了再现性和降低了变异性。采用毛细管电泳技术进行DNA测序是人类基因组计划得以完成的技术突破。毛细管电泳在蛋白质表征中的应用有望彻底改变传统的免疫印迹方法。该仪器将放置在位于VAPSHCS，西雅图1号楼710室的分子遗传学核心机构中;因此，所有研究者均可使用该仪器，作为付费使用服务。仪器本身将由经过培训的核心工作人员操作。该仪器的主要用户包括Steven Kahn、大卫库克、Chang-en Yu、Nicole Liachko和Brian Kraemer;次要用户将是William Banks和Rebecca船体。总的来说，该仪器可用于任何专注于蛋白质的分子研究，并有可能取代手动Western印迹和ELISA分析。该仪器的整体购买将有望提高VA PSHCS作为研究机构的竞争力，并进一步加强其研究人员与蛋白质分子工作的有效性和效率。该仪器将通过将免疫印迹分析的成本降低高达60%，从而在节省的时间和劳动力方面将其成本提高许多倍。因此，研究小组将释放资源，更迅速、更深入地追求他们的目标，从而使创伤性脑损伤、糖尿病、痴呆和肌萎缩性侧索硬化症的研究取得更快的进展。