Acquisition of a ProteinSimple capillary electrophoresis protein immunoanalyzer

购置 ProteinSimple 毛细管电泳蛋白质免疫分析仪

• 批准号: 8949379
• 负责人: Brian C. Kraemer
• 金额: --
• 依托单位: VA PUGET SOUND HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2015-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8949379
• 关键词: Acrylamides; Address; Adoption; Amyotrophic Lateral Sclerosis; Antibodies; Area; Biological Assay; Blood capillaries; Caliber; Capillary Electrophoresis; Cataloging; Catalogs; Computer software; Computers; Consumption; Core Facility; Cost Effectiveness Analysis; DNA Sequence; Data; Data Analyses; Dementia; Detection; Diabetes Mellitus; Effectiveness; Electrophoresis; Enzyme-Linked Immunosorbent Assay; Fees; Hand; Hour; Housing; Human Genome Project; Immobilization; Immunoblot Analysis; Immunoblotting; Individual; Institution; Investigation; Laboratories; Legal patent; Length; Licensing; Manuals; Materials Testing; Methodology; Methods; Minor; Molecular; Molecular Genetics; Molecular Weight; Monitor; Process; Protein Analysis; Proteins; Reagent; Reproducibility; Research; Research Personnel; Research Priority; Resources; Sampling; Services; Signal Transduction; Sodium Dodecyl Sulfate; Standardization; System; Technology; Time; Training; Translational Regulation; Traumatic Brain Injury; Western Blotting; Work; assay development; base; capillary; computerized data processing; cooking; cost; design; instrument; meetings; nanoimmunoassay; nanolitre; preference; programs; public health relevance; research study

 DESCRIPTION (provided by applicant): Request for a ProteinSimple Instrument. We are requesting the purchase of a ProteinSimple capillary electrophoresis protein immunoanalysis system (aka SallySue) for the high throughput cost effective analysis of proteins. This instrument utilizes capillary electrophoretic technology o separate proteins by size like standard sodium dodecyl sulfate poly acrylamide electrophoresis methodology. Subsequent immunodetection functions much like standard chemiluminescent immunoblotting approaches. The main advantages of the SallySue instrument over manual approaches are dramatically reduced hands on time, increased throughput, reduced materials cost, reduced sample consumption, increased quantitative accuracy, increased reproducibility, and lower variability. Adoption of capillary electrophoresis technology for DNA sequencing was the technological breakthrough permitting completion of the human genome project. Application of capillary electrophoresis to protein characterization promises to revolutionize conventional immunoblotting methods. This instrument will be placed in the Molecular Genetics Core facility located in Building 1, Room 710 at VAPSHCS, Seattle; as such it will be available for use by all investigators as a fee for use service. The instrument itself will be operated by trained core staf. Major users of the instrument include Steven Kahn, David Cook, Chang-en Yu, Nicole Liachko, and Brian Kraemer; minor users will be William Banks and Rebecca Hull. Overall, the instrument has utility for any molecular investigation focused on protein and has the potential to replace manual western blotting and ELISA analysis. Overall purchase of this instrument will be expected to increase the competitiveness of VA PSHCS as a research institution and further strengthen the effectiveness and efficiency of its investigators molecular work with protein. The instrument will return its cost many times over in terms of saved time and labor by reducing costs for immunoblotting analysis by up to 60%. Thus research teams will have freed up resources to pursue their objectives more rapidly and in greater depth allowing more rapid progress in research into traumatic brain injury, diabetes, dementia and amyotrophic lateral sclerosis.

 描述(由申请人提供)： 申请ProteinSimple仪器。我们要求购买蛋白质简单毛细管电泳蛋白质免疫分析系统(又名SallySue)，用于高通量、高性价比的蛋白质分析。该仪器利用毛细管电泳法分离蛋白质，就像标准的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法。随后的免疫检测功能很像标准的化学发光免疫印迹方法。与人工方法相比，SallySue仪器的主要优势是大大减少了实际操作时间，增加了产量，降低了材料成本，减少了样品消耗，提高了定量准确性，增加了重复性，并降低了变异性。采用毛细管电泳法进行DNA测序是完成人类基因组计划的技术突破。毛细管电泳法在蛋白质鉴定中的应用有望彻底改变传统的免疫印迹方法。该仪器将放置在位于西雅图VAPSHCS的1号楼710室的分子遗传学核心设施中；因此，所有研究人员都可以使用它作为使用服务费。仪器本身将由训练有素的核心STAF操作。该乐器的主要用户包括史蒂文·卡恩、大卫·库克、余昌恩、妮可·利亚奇科和布莱恩·克莱默；次要用户将是威廉·班克斯和丽贝卡·赫尔。总体而言，该仪器对任何以蛋白质为重点的分子研究都是有用的，并有可能取代人工的Western blotting和ELISA分析。对这台仪器的整体购买预计将增加VA PSHCS作为研究机构的竞争力，并进一步加强其研究人员在蛋白质分子工作中的效力和效率。通过将免疫印迹分析的成本降低高达60%，该仪器将在节省时间和劳动力方面实现成倍的回报。因此，研究团队将腾出资源，更快、更深入地追求他们的目标，从而使创伤性脑损伤、糖尿病、痴呆症和肌萎缩侧索硬化症的研究取得更快的进展。