RUI: Acquistion of a Fluorescence/PhosphorImager - MD STORM 860

RUI：购买荧光/磷光成像仪 - MD STORM 860

• 批准号: 0070401
• 负责人: Anne Hitt
• 金额: $ 5.93万
• 依托单位: Oakland University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070401&HistoricalAwards=false
• 关键词: RUI; Acquistion; Fluorescence; PhosphorImager; MD

Abstract HittThe first step toward understanding any biological system is to identify and quantify its components. The second step is to determine the interactions between the components of a system. A fluorescence/phosphorimager can provide the technology needed to begin to accomplish these steps. The STORM 860 Fluorescence/Phosphorimager will be used to aid in the identification and quantification of molecules, e.g., proteins, DNA, RNA, of interest. In addition, it will be used to visualize and analyze molecular interactions between well-characterized molecules and previously uncharacterized molecules. The imager will be used to address fundamental questions in biology including - investigations into protein structure and function, regulation of fatty acid biosynthesis, regulation of RNA polymerase assembly, analysis of recombinant protein expression by RNAse protection assays, and gene expression analysis by screening cDNA microarrays.Because the fluorescence/phosphorimager imaging and analysis technology is very robust with wide-ranging applications for cellular, molecular and biochemical research programs, it will strengthen and expand active research programs at Oakland University. Furthermore, the imager will strengthen and expand the training and education of university students, teachers, and other working professionals in biochemistry, cell and molecular biology.

理解任何生物系统的第一步是识别和量化它的组成部分。第二步是确定系统组件之间的相互作用。荧光/磷成像仪可以提供开始完成这些步骤所需的技术。STORM 860荧光/磷光成像仪将用于帮助识别和量化感兴趣的分子，例如蛋白质、DNA、RNA。此外，它还将用于可视化和分析表征良好的分子和以前未表征的分子之间的分子相互作用。该成像仪将用于解决生物学中的基本问题，包括蛋白质结构和功能的研究，脂肪酸生物合成的调控，RNA聚合酶组装的调控，核糖核酸酶保护分析重组蛋白的表达，以及通过筛选cDNA微阵列进行基因表达分析。由于荧光/磷成像仪成像和分析技术非常强大，可广泛应用于细胞、分子和生化研究项目，它将加强和扩大奥克兰大学的积极研究项目。此外，该成像仪将加强和扩大对大学生、教师和其他工作人员在生物化学、细胞和分子生物学方面的培训和教育。