Dissertation Research: Intraspecific Phylogenetics of a Crayfish: Testing a Nested Analysis of Population Structure in a one Dimensional Habitat.

[00:73155]有几种现象可以导致目前在自然种群中观察到的遗传模式。在这些不同的现象中进行选择的一种强有力的方法是嵌套分析程序。虽然这种方法已成功地应用于几种生物，但它只适用于陆地生物。当该方法应用于占据水生生境的生物时，可能需要对其进行某些修改。本研究的目的是检验水生生物案例的巢式分析程序，并概述分析这些类型种群数据的最合适方法。这项研究将利用从密苏里州收集的60种不同小龙虾种群的DNA序列来测试修改水生生物程序的适宜性。该程序将进行两种变化：1)标准方法（地理坐标）和2)用于水生生物的修改（河内距离）。然后将比较两种方法的结果，以确定推断现象的显著偏差。这项研究将允许巢式分析程序适当地扩展到水生生物。如果没有这样的研究，使用标准方法对水生生物的遗传结构所作的推断可能会产生误导。

0073155CrandallThere are several phenomena that can lead to currently observed genetic patterns in natural populations. One powerful method that has been developed to choose among these various phenomena is the nested-analysis procedure. While this method has been utilized successfully in several organisms it has only been applied to land dwelling organisms. Certain modifications to the methodology may be needed when it is applied to organisms occupying aquatic habitats. The goals of this study are to examine the nested-analysis procedure for the case of aquatic organisms and outline the most appropriate ways to analyze data from these types of populations. This study will utilize DNA sequences from 60 different crayfish populations collected from throughout Missouri to test the appropriateness of modifying the procedure for aquatic organisms. Two variations of the procedure will be conducted: 1) the standard methodology (geographic coordinates) and 2) a modification for use with aquatic organisms (in-river distances). Results of the two methods will then be compared for significant deviations in the inferred phenomena. This study will allow for the proper extension of the nested-analysis procedure to aquatic organisms. Without such a study, the inferences made about the genetic structure of aquatic organisms using standard methodologies may be misleading.