Genetic Screens for Drosophila p70 S6 Kinase Modifiers

果蝇 p70 S6 激酶修饰剂的遗传筛选

基本信息

  • 批准号:
    0077618
  • 负责人:
  • 金额:
    $ 25万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2000
  • 资助国家:
    美国
  • 起止时间:
    2000-07-15 至 2004-06-30
  • 项目状态:
    已结题

项目摘要

Stewart 0077618AbstractThe mammalian p70S6 kinase, termed S6K1, is a mitogen-stimulated serine/threonine kinase that lies on a signaling pathway leading to the control of translation and cell growth. The current model for S6K1 function is that upon activation, S6K1 phosphorylated the 40S ribosomal subunit protein S6 and this causes the ribosome to selectively increase the translation of a group of mRNAs that encode ribosomal proteins and translation initiation factors. This series of events would be expected to increase ribosome biogenesis and the translational capacity of the cell and thus allow increased general protein synthesis and cell growth. The activation of S6K1 is complex and is associated with the phosphorylation of eight amino acids in the kinase. The signaling pathways that lead to S6K1 activation are only partially defined and only the identity of one of the kinases that phosphorylates one of the eight phosphorylated amino acids inS6K1 that are associated with the activation of the kinase is known. The Drosophila melanogaster S6 kinase homolog, dS6K, is structurally and functionally conserved with the mammalian S6K1. In cultured cells, dS6K can be activated or inhibited by agents that activate or inhibit S6K1, suggesting that the mechanisms that regulate dS6K and S6K1 activation will be conserved between Drosophila and mammals. Similar to the proposed role for S6K1 in translation and growth, the role for dS6K within the fly appears to be in regulating cell size, cell growth and cell proliferation. The overall goal of this proposal is to use the power of Drosophila genetics to identify novel components of the dS6K pathway. Reaching this goal will entail:1. Testing if mutant alleles of genes that encode suspected components of the dS6K signaling pathway interact genetically with mutant dS6K alleles2. Screening a collection of Drosophila deficiency stocks to identify dose-sensitive modifiers of dS6K mutant phenotypes3. Performing mutagenesis screens designed to identify newly induced mutations in genes that encode components of the dS6K signaling pathways.Identifying components of the dS6K signaling pathways will be important for increasing our knowledge of intracellular signaling and also should impact our understanding of the basic mechanisms that regulate translation and cell growth.
Stewart 0077618摘要哺乳动物 p70S6 激酶,称为 S6K1,是一种有丝分裂原刺激的丝氨酸/苏氨酸激酶,位于导致控制翻译和细胞生长的信号传导通路上。目前 S6K1 功能的模型是,激活后,S6K1 磷酸化 40S 核糖体亚基蛋白 S6,这导致核糖体选择性增加一组编码核糖体蛋白和翻译起始因子的 mRNA 的翻译。这一系列事件预计将增加核糖体生物发生和细胞的翻译能力,从而增加一般蛋白质合成和细胞生长。 S6K1 的激活很复杂,与激酶中八个氨基酸的磷酸化有关。导致 S6K1 激活的信号传导途径仅部分确定,并且仅已知磷酸化 S6K1 中与激酶激活相关的八个磷酸化氨基酸之一的激酶之一。果蝇 S6 激酶同源物 dS6K 在结构和功能上与哺乳动物 S6K1 一样保守。在培养细胞中,dS6K 可以被激活或抑制 S6K1 的试剂激活或抑制,这表明调节 dS6K 和 S6K1 激活的机制在果蝇和哺乳动物之间是保守的。与 S6K1 在翻译和生长中的拟议作用类似,果蝇中 dS6K 的作用似乎是调节细胞大小、细胞生长和细胞增殖。该提案的总体目标是利用果蝇遗传学的力量来识别 dS6K 通路的新成分。实现这一目标需要:1.测试编码 dS6K 信号通路可疑成分的基因突变等位基因是否与突变 dS6K 等位基因发生遗传相互作用2。筛选果蝇缺陷种群的集合,以确定 dS6K 突变表型的剂量敏感修饰剂3。进行诱变筛选,旨在识别编码 dS6K 信号通路成分的基因中新诱导的突变。识别 dS6K 信号通路成分对于增加我们对细胞内信号传导的了解非常重要,也应该影响我们对调节翻译和细胞生长的基本机制的理解。

项目成果

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Mary Stewart其他文献

Impacts from Resource Use - A common position paper
Alcohol versus natural drying for newborn cord care.
酒精与自然干燥用于新生儿脐带护理。
User needs analysis and development of priorities for life cycle impact assessment
Risk factors for hepatitis C virus infection among injecting drug users in Sydney.
悉尼注射吸毒者感染丙型肝炎病毒的危险因素。
  • DOI:
  • 发表时间:
    1994
  • 期刊:
  • 影响因子:
    0
  • 作者:
    I. Beek;R. Buckley;Mary Stewart;M. MacDonald;John M. Kaldor
  • 通讯作者:
    John M. Kaldor
11-OR: Initial results of the NMDP high resolution KIR typing project
  • DOI:
    10.1016/j.humimm.2006.08.016
  • 发表时间:
    2006-10-01
  • 期刊:
  • 影响因子:
  • 作者:
    Raja Rajalingam;Harriet Noreen;Neng Yu;Rebecca Cullen;Zeying Du;Lihui Luo;Maurine Davidson;Mary Stewart;Tatiana Lebedeva;Marina Ohashi;Stephen Spellman;Elaine Reed
  • 通讯作者:
    Elaine Reed

Mary Stewart的其他文献

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{{ truncateString('Mary Stewart', 18)}}的其他基金

Ageing, Health, and Social Care: The meaningful engagement of autistic people with learning disabilities in supported living services in Scotland
老龄化、健康和社会关怀:苏格兰有学习障碍的自闭症患者有意义地参与支持性生活服务
  • 批准号:
    AH/X012573/1
  • 财政年份:
    2023
  • 资助金额:
    $ 25万
  • 项目类别:
    Research Grant
A Program to Award Dissertation Fellowships in Hazards, Risk, and Disasters
授予灾害、风险和灾害论文奖学金的计划
  • 批准号:
    0927317
  • 财政年份:
    2009
  • 资助金额:
    $ 25万
  • 项目类别:
    Standard Grant
Tulsa Public Schools Mathematics/Science Systemic Reform Initiative
塔尔萨公立学校数学/科学系统改革倡议
  • 批准号:
    9817614
  • 财政年份:
    1999
  • 资助金额:
    $ 25万
  • 项目类别:
    Standard Grant
Powre: Analysis of p70 S6 Kinase in Drosophila
Power: 果蝇中 p70 S6 激酶的分析
  • 批准号:
    9806127
  • 财政年份:
    1998
  • 资助金额:
    $ 25万
  • 项目类别:
    Standard Grant

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