Powre: Analysis of p70 S6 Kinase in Drosophila

摘要9806127斯图尔特细胞生长的控制涉及许多过程，其中蛋白质合成是核心。这项研究的总体目标是了解导致蛋白质合成和细胞生长调节的细胞信号机制。果蝇将被用作这些研究的模型系统，因为参与蛋白质合成的许多细胞成分在果蝇和哺乳动物之间高度保守。在哺乳动物细胞中，p70核糖体蛋白S6激酶，p70 S6K，存在于细胞信号通路上，最终导致蛋白质合成的改变。最近发现了果蝇p70核糖蛋白S6蛋白的同源物和影响该基因的突变。将对该基因进行定点突变，并产生携带该基因突变版本的转基因果蝇。这项POWRE奖将允许PI学习和建立转化所需的胚胎显微注射技术，并进行中试实验。*这是一项POWRE奖，它将使研究人员能够学习和建立必要的技术，使转基因果蝇表达在原生动物合成和其他细胞过程中至关重要的蛋白质突变。这将使进一步的实验能够确定它的作用。

Abstract 9806127 Stewart The control of cell growth involves a number of processes to which protein synthesis is central. The broad term objectives of this research are to understand the mechanisms of cell signaling that lead to the regulation of protein synthesis and cell growth. Drosophila melanogaster will be used as a model system in these studies since many cellular components involved in protein synthesis are highly conserved between flies and mammals. In mammalian cells, the p70 ribosomal protein S6 kinase, p70 S6K, lies on a cellular signaling pathway that ultimately leads to alterations in protein synthesis. The Drosophila p70 ribosmal protein S6kinase homologue and mutations affecting this gene have recently been identified. Site directed mutations of this gene will be made and transgenic flies bearing mutant versions of this gene will be generated. This POWRE award will permit the PI to learn and establish the technique of microinjection into embryos required for transformation, and to perform pilot experiments. *** This is a POWRE award which will enable the investigator to learn and establish the necessary techniques to make transgenic flies expressing mutants of a protein critical in protozoon synthesis and other cellular processes. This will enable further experiments to determine it role.