RUI: Collaborative Proposal: Biochemical and Physiological Adaptation to Temperature in a Montane Insect Herbivore

RUI：合作提案：山地昆虫草食动物对温度的生化和生理适应

• 批准号: 0078464
• 负责人: Elizabeth Dahlhoff
• 金额: $ 20.34万
• 依托单位: Santa Clara University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0078464&HistoricalAwards=false
• 关键词: RUI; Collaborative; Proposal; Biochemical; Physiological

This research investigates evolution in high elevation populations of the leaf beetle Chrysomela aeneicollis. Populations in the Sierra Nevada (California) are on the southern edge of the species' range. Previous studies found that drainages differ in air temperature and beetle populations fluctuate with changes in climate. Frequencies of different forms (genotypes) of the temperature-sensitive enzyme phosphoglucose isomerase (PGI) vary among drainages, suggesting that natural selection acts on PGI. Heat shock protein (Hsp) levels in beetles correspond to air temperature differences and depend on PGI genotype. The proposed research will investigate the relationship between temperature and functional property of PGI genotypes (i.e. their ability to catalyze biochemical reactions fundamental to metabolism). Second, the effects of temperature on the physiology of beetles of different PGI genotype will be studied, using Hsp's and ubiquitin conjugates as indices of physiological stress. Third, survival and reproduction of beetles of different PGI genotype in nature will be compared. The research will provide important information about the evolutionary significance of enzyme polymorphisms. It may add insight about the impact of climate change on native insects. Finally, it will allow undergraduates to participate in basic research, enhance their educational experience and expand their opportunities for the future

这项研究调查了叶甲虫 Chrysomela aeneicollis 高海拔种群的进化。 内华达山脉（加利福尼亚州）的种群位于该物种分布范围的南端。 先前的研究发现，排水系统因气温而异，甲虫的数量也会随着气候的变化而波动。 温度敏感酶磷酸葡萄糖异构酶 (PGI) 的不同形式（基因型）的频率在不同排水系统中存在差异，表明自然选择对 PGI 起作用。 甲虫中的热休克蛋白 (Hsp) 水平与气温差异相对应，并取决于 PGI 基因型。 拟议的研究将调查温度和 PGI 基因型功能特性（即它们催化新陈代谢基础生化反应的能力）之间的关系。 其次，将使用热休克蛋白和泛素结合物作为生理应激指标，研究温度对不同 PGI 基因型甲虫生理的影响。 第三，比较不同PGI基因型甲虫在自然界中的生存和繁殖。 该研究将为酶多态性的进化意义提供重要信息。 它可能会增加人们对气候变化对本土昆虫影响的了解。 最后，它将使本科生能够参与基础研究，增强他们的教育经验并扩大他们未来的机会