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RUI: Collaboration: Biochemical Adaptation in a Montane Insect Herbivore

RUI：合作：山地昆虫草食动物的生化适应

基本信息

批准号：
9808836
负责人：
Elizabeth Dahlhoff
金额：
$ 2.27万
依托单位：
Santa Clara University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1998
资助国家：
美国
起止时间：
1998-07-15 至 2000-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9808836&HistoricalAwards=false
关键词：
RUI Collaboration Biochemical Adaptation Montane

项目摘要

Dahlhoff 9808836 The PIS will examine mechanisms of temperature adaptation in the leaf beetle Chrysomela aerteicollis living in the Eastern Sierra Nevada, California. The PIs have selected beetle populations living in three drainages characterized by steep altitudinal and thermal gradients: Big Pine Creek, Bishop Creek, and Rock Creek. The PIs will characterize the thermal environment of these drainages and determine the body temperatures of beetles living in a variety of microhabitats. Air temperatures will be measured continuously over the summer at three altitudes per drainage. The PIs will map sunny and shady habitats in each locality precisely. The PIs will use digital thermometers equipped with micro-thermocouples to measure the body temperatures of adult beetles and larvae over the course of the summer: at a single location during the day,at different altitudes over several days, and in sunny and shady sites in each locality. The PIs will also quantify tissue levels of the heat-stress inducible protein HSP70 in beetles of known body temperature- Finally, the PIs will use enzyme electrophoresis to characterize the populations genetically. These results will allow us to quantify environmental temperature variation and the beetles' response to it. Both field and laboratory portions of this project will involve extensive participation of undergraduates.

达尔霍夫9808836 PIS将研究生活在加州东塞拉内华达州的叶甲虫Aerteicollis的温度适应机制。 PI选择了生活在三个具有陡峭海拔和温度梯度的排水系统中的甲虫种群：大松溪，主教溪和岩溪。 PI将描述这些排水系统的热环境，并确定生活在各种微生境中的甲虫的体温。在夏季，每个排水系统将在三个海拔高度连续测量气温。PI将精确地绘制每个地方的阳光和阴凉栖息地。PI将使用配备微型热电偶的数字温度计在整个夏季测量成年甲虫和幼虫的体温：白天在一个地点，几天内在不同海拔高度，以及每个地方的阳光和阴凉地点。PI还将量化已知体温的甲虫中热应激诱导蛋白HSP 70的组织水平-最后，PI将使用酶电泳来表征种群的遗传特征。这些结果将使我们能够量化环境温度变化和甲虫对它的反应。这个项目的野外和实验室部分将涉及大学生的广泛参与。