Self Assembly of Helical Cyanine Dye Aggregates on Nucleic Acid Templates

核酸模板上螺旋花青染料聚集体的自组装

• 批准号: 0078765
• 负责人: Bruce Armitage
• 金额: $ 30.9万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0078765&HistoricalAwards=false
• 关键词: Self; Assembly; Helical; Cyanine; Dye

This project will explore the controlled aggregation of cyanine dyes on nucleic acid nanotemplates. The end result is the spontaneous assembly of chiral, helical aggregates of cyanine dimers. The dimensions of the aggregate are controlled by varying the length of the DNA. The properties of the aggregates will be studied using a variety of spectroscopic techniques, including UV-vis absorption, circular dichroism and fluorescence methods, and by stopped flow spectroscopy. Collaborative efforts will explore the possibilities of X-ray crystallographic studies of these aggregates, as well as a theoretical treatment of the electronic interactions among the cyanine dyes.With this award, the Organic and Macromolecular Chemistry Program's Organic Synthesis Program supports the research of Professor Bruce A. Armitage of Carnegie Mellon University. Professor Armitage will investigate the properties of certain dye molecules to assemble on a template consisting of the DNA helix. Thus assembled, these aggregates can themselves serve as the template for the generation of complex structures that retain some of the characteristics initially imparted by the DNA. The properties of these assemblies will be studied as will the dynamics of their formation.

该项目将探索花青染料在核酸纳米模板上的受控聚集。 最终结果是花青二聚体的手性螺旋聚集体的自发组装。 聚集体的尺寸通过改变 DNA 的长度来控制。 将使用各种光谱技术来研究聚集体的性质，包括紫外可见吸收、圆二色性和荧光方法以及停流光谱法。 合作将探索这些聚集体的 X 射线晶体学研究的可能性，以及花青染料之间电子相互作用的理论处理。有机和高分子化学项目的有机合成项目通过该奖项支持卡内基梅隆大学 Bruce A. Armitage 教授的研究。 Armitage 教授将研究某些染料分子在由 DNA 螺旋组成的模板上组装的特性。 通过组装，这些聚集体本身可以作为生成复杂结构的模板，这些结构保留了 DNA 最初赋予的一些特征。 将研究这些组件的特性及其形成的动力学。