Novel Techniques for the Solution and Solid Phase Synthesis of Peptides and Peptide Segments

本项目涉及制备较长肽和小蛋白质的方法，其中主要缺陷出现在当前方法应用于耦合步骤中。辅助关注的是诸如组氨酸和精氨酸等氨基酸残基的保护基团，特别是在涉及货架稳定性的情况下。注意力将指向在酸性脱保护步骤中不释放阳离子的新的精氨酸保护基团。偶联策略包括发现与溶液相容的试剂以及固相肽合成。有了这个奖项，有机和大分子化学项目的有机合成项目支持了马萨诸塞大学阿默斯特分校路易斯·a·卡皮诺教授的研究。Carpino教授探索如何将相对较小的氨基酸组合成更大的组合，即肽和蛋白质。它们在生物技术领域具有深远的影响，但它们的合成往往相当困难。随着对多肽探测生物学研究问题需求的不断增长，这些研究具有现实意义。

This project deals with methods to prepare longer peptides and small proteins, where a major deficiency appears in the application of current methods to the coupling steps. An ancillary concern are protective groups for such amino acid residues as histidine and arginine, particularly where shelf-stability is concerned. Attention will be directed toward new arginine protective groups that do not release cationic species during acidic deprotection steps. Coupling strategies include the discovery of reagents that are compatible with solution as well as solid phase peptide synthesis.With this Award, the Organic and Macromolecular Chemistry Program's Organic Synthesis Program supports the research of Professor Louis A. Carpino of the University of Massachusetts, Amherst. Professor Carpino explores ways to combine relatively small groups of amino acids into larger assemblages known as peptides and proteins. These have profound implications in the area of biotechnology, but their synthesis is often quite difficult. With the continued growth in the demand for peptides to probe biological research questions, these studies are of practical significance.