Novel Techniques for the Solution and Solid Phase Synthesis of Peptides and Peptide Segments

肽和肽片段的溶液和固相合成新技术

• 批准号: 0078971
• 负责人: Louis Carpino
• 金额: $ 38.5万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0078971&HistoricalAwards=false
• 关键词: Novel; Techniques; Solution; Solid; Phase

This project deals with methods to prepare longer peptides and small proteins, where a major deficiency appears in the application of current methods to the coupling steps. An ancillary concern are protective groups for such amino acid residues as histidine and arginine, particularly where shelf-stability is concerned. Attention will be directed toward new arginine protective groups that do not release cationic species during acidic deprotection steps. Coupling strategies include the discovery of reagents that are compatible with solution as well as solid phase peptide synthesis.With this Award, the Organic and Macromolecular Chemistry Program's Organic Synthesis Program supports the research of Professor Louis A. Carpino of the University of Massachusetts, Amherst. Professor Carpino explores ways to combine relatively small groups of amino acids into larger assemblages known as peptides and proteins. These have profound implications in the area of biotechnology, but their synthesis is often quite difficult. With the continued growth in the demand for peptides to probe biological research questions, these studies are of practical significance.

该项目涉及制备较长肽和小蛋白质的方法，其中当前方法在偶联步骤中的应用存在重大缺陷。 一个辅助问题是组氨酸和精氨酸等氨基酸残基的保护基团，特别是在考虑储存稳定性的情况下。 注意力将被引导到新的精氨酸保护基团，不释放阳离子物种在酸性脱保护步骤。 偶联策略包括发现与溶液相容的试剂以及固相肽合成。有了这个奖项，有机和大分子化学计划的有机合成计划支持路易斯·A·马萨诸塞州大学阿默斯特分校的卡尔皮诺说。 卡皮诺教授探索了将相对小的氨基酸组联合收割机组合成更大的集合体（称为肽和蛋白质）的方法。 这些在生物技术领域具有深远的影响，但它们的综合往往相当困难。 随着对多肽的需求的不断增长，这些研究具有现实意义。