Comprehensive Upgrade of a Shared Campus Nuclear Magnetic Resonance Facility

该奖项将资助用于生物大分子和天然产物的最先进的核磁共振（NMR）研究的冷冻探针。低温探针将在加州大学戴维斯分校的核磁共振设施中被化学、生物化学和结构生物学领域的研究人员使用。具体的例子包括研究蛋白质、核酸、碳水化合物、脂类和各种来源的天然产物的分子结构和动力学。高灵敏度低温探针大大提高了实验灵敏度，减少了实验运行时间，降低了研究所需的最小样本量，从而开辟了新的生产研究领域。低温探头封装包括低温冷却的核磁共振探头，安装平台，相关的低温泵和管道，以及低温冷却的前置放大器。探测器将是三重共振型，具有质子观测和碳氮解耦通道。一个z场梯度线圈也是探针的一部分。对于相同的样品体积和浓度，探针的灵敏度大约是非低温探针的两到四倍。这种增加转化为运行时间减少了大约4到16倍。如此大幅度的节省运行时间，有效地以一小部分成本提供了另一个完整的光谱仪。该探针将能够进行生物大分子和天然产物的多共振、多维溶液研究。该仪器将成为UCD核磁共振设施的一部分。该设施每年由40多名教职员工（在戴维斯校区的大约20个部门）使用。这些研究小组有大约140名学生和50名博士后研究员，其中大约40人是仪器的常规用户。核磁共振光谱仪是每年14个班级的辅助部分，平均总招生人数超过100人。光谱仪也为学生和教师招聘提供了最先进的资源。因此，冷冻探针将对加州大学戴维斯分校的研究和教育基础设施做出重大贡献。

This award will fund a cryoprobe for state-of-the-art nuclear magnetic resonance (NMR) studies of biological macromolecules and natural products. The cryoprobe will be used at the UC Davis NMR Facility by researchers in the fields of chemistry, biochemistry, and structural biology. Specific examples include studies of the molecular structure and dynamics of proteins, nucleic acids, carbohydrates, lipids, and natural products from a variety of sources. The high sensitivity cryoprobe dramatically increases experimental sensitivity, reduces experimental run times, and lowers the minimum amount of sample needed for study, thus opening new areas of productive research. The cryoprobe package consists of a cryogenically cooled NMR probe, a mounting platform, associated cryogenic pumps and tubing, and a cryogenically cooled preamplifier. The probe will be a triple resonance type, with proton observation and both carbon and nitrogen decoupling channels. A Z-field gradient coil is also part of the probe. The probe sensitivity is approximately two to four times that of non-cryogenic probes for the same sample volume and concentration. This increase translates into reductions in run time of approximately four to sixteen fold. Such a drastic savings in run time effectively provides another entire spectrometer at a fraction of the cost. The probe will be capable of multi-resonance, multi-dimensional solution studies of biological macromolecules and natural products. The instrumentation will become part of the UCD NMR Facility. The Facility is utilized annually by over 40 faculty members (in approximately 20 departments of the Davis campus). These research groups have approximately 140 students and 50 postdoctoral fellows, of whom approximately 40 individuals are regular hands-on users of the instrumentation. The NMR spectrometers are an ancillary part of 14 classes annually with an average total enrollment of over 100 students. The spectrometers also serve as a state of the art resource for student and faculty recruitment. The cryoprobe will thus make a significant contribution to the research and educational infrastructure at the University of California, Davis.