Acquisition of Equipment for Genomics at Auburn University

高通量自动化核苷酸测序对于基因组学研究至关重要。 奥本大学的一系列研究项目需要广泛的DNA测序和片段分析。 这些项目包括分子标记的开发、遗传作图、标记辅助选择、遗传资源分析、种质保存、分子生态学、群体遗传学、系统发育研究、分子进化、基因发现和克隆、分子检测、环境基因组学和功能基因组学。 该奖项将支持高通量DNA分析设备的收购，这将大大提高奥本的研究社区的能力。 ABI Prism 377自动DNA测序仪是一种高通量机器，每次运行可检测96个样品。 它比基于毛细管的DNA测序仪更容易使用，因此更适合于有多个用户的核心设施。 它的收购将满足奥本大学和该地区目前和不断增长的DNA分析需求。 该奖项支持的仪器将解决该地区研究需求的一个关键方面。 它将大大提高研究能力、效率、精度和产出。 应用从加速研究、研究培训和推广中获得的信息将对亚拉巴马产生巨大的经济影响。购买这些设备将是改善奥本大学研究基础设施的重要一步。 大量的研究生和本科生将受益于他们参与研究项目使用的设备或课程提供实践经验和先进的基因组技术研讨会。 这些设备也将提供给塔斯基吉大学的研究人员和学生。

High throughput automated nucleotide sequencing is critically important for genomics research. A wide array of research projects at Auburn University requires extensive DNA sequencing and fragment analysis. These projects include development of molecular markers, genetic mapping, marker-assisted selection, genetic resource analysis, germplasm conservation, molecular ecology, population genetics, phylogenetic studies, molecular evolution, gene discovery and cloning, molecular detection, environmental genomics, and functional genomics. This award will support the acquisition of high throughput DNA analysis equipment that will greatly increase the capacity of Auburn's research community. The ABI Prism 377 automated DNA sequencer is a high throughput machine with a capacity of 96 samples per run. It is easier to use than the capillary-based DNA sequencers and, therefore, is more suitable for a core facility with multiple users. Its acquisition will meet the current and growing needs in DNA analysis at Auburn University and in the region. The instrumentation supported by this award will address a critical aspect of research need in the region. It will dramatically enhance research capacity, efficiency, precision, and output. Application of the information obtained from accelerated research, research training, and outreach will have a large economic impact in Alabama. Acquisition of this equipment will represent an important step toward improving the research infrastructure at Auburn University. A large number of graduate and undergraduate students will benefit by their involvement in research projects using the equipment or from course offerings providing hands-on experience and workshops on advanced technologies in genomics. The equipment will also be made available to researchers and students at Tuskegee University.