MRI: Acquisition of equipment for microbial single cell genomics research

MRI：购置微生物单细胞基因组学研究设备

• 批准号: 0821374
• 负责人: Ramunas Stepanauskas
• 金额: $ 49.4万
• 依托单位: Bigelow Laboratory for Ocean Sciences
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-15 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0821374&HistoricalAwards=false
• 关键词: MRI; Acquisition; equipment; microbial; single

Intellectual Merit:The PIs propose to acquire instrumentation that will form the basis for a new microbial single cell genomics facility at the institution. Single cell genomics represents a novel approach to study uncultured microorganisms and serves as a complement to metagenomics. The proposed instrumentation will enable research efforts investigating biogeochemistry and microbial interactions, microbial evolution and diversity, and biotechnology. The proposed instrumentation includes: nanoliter reagent dispenser, a thermal plate sealer, a PCR hood, an anaerobic chamber, a liquid handling workstation, a real-time PCR thermocycler, an ultracold freezer, and a bioinformatics computer cluster. The requested instrumentation would enable high throughput wetlab and computational DNA analyses and help reduce DNA contamination and overall per-sample costs, two of the most significant limitations for single cell genomics to date. The new facility will be operated in close collaboration with an existing aquatic cytometry facility using established user fees, scheduling and protocols. The lead PI will be responsible for the overall management of the purchase, installation, and operation of the instrumentation. A part-time technician will be employed for the wet lab instrumentation. The instrumentation will initially be located in different laboratories, but will be relocated to a dedicated workspace upon construction of new laboratory facilities at the institution projected for 2010. Broader Impacts:In addition to establishing a shared-use facility to enable access to single cell genomics techniques, the PIs propose developing an annual course in single cell DNA analysis, as well as incorporating classes on single cell genomics into existing courses. The PIs will also encourage on-site visits by college and university faculty and students.

智力优势：PI建议获得仪器，这将成为该机构新的微生物单细胞基因组学设施的基础。 单细胞基因组学是研究未培养微生物的一种新方法，是宏基因组学的补充。 拟议的仪器将使研究工作调查生物地球化学和微生物的相互作用，微生物的进化和多样性，以及生物技术。 建议的仪器包括：纳升试剂分配器，热板蒸发器，PCR罩，厌氧室，液体处理工作站，实时PCR热循环仪，超冷冷冻机，和生物信息学计算机集群。 所要求的仪器将实现高通量湿实验室和计算DNA分析，并有助于减少DNA污染和总体每份样品成本，这是迄今为止单细胞基因组学的两个最重要的限制。新设施将与现有的水生细胞计数设施密切合作，使用既定的用户费用、时间安排和协议。 首席PI将负责仪器的采购、安装和操作的全面管理。 将雇用一名兼职技术人员负责湿实验室仪器。 这些仪器最初将设在不同的实验室，但预计在2010年在该机构建造新的实验室设施后，将搬迁到一个专用工作空间。更广泛的影响：除了建立一个共享使用的设施，使人们能够获得单细胞基因组学技术，PI建议开发一个单细胞DNA分析的年度课程，以及将单细胞基因组学课程纳入现有课程。 PI还将鼓励学院和大学的教职员工和学生进行现场访问。