Signal Pathways-Mediated by the Rice Receptor-Like Kinase XA21

水稻受体样激酶 XA21 介导的信号通路

• 批准号: 0080155
• 负责人: Wen-Yuan Song
• 金额: $ 37.5万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0080155&HistoricalAwards=false
• 关键词: Signal; Pathways; Mediated; Rice; Receptor

The long-term goal of this project is to gain an understanding of receptor serine/threonine kinase-mediated signaling in plants. Receptor kinases, including receptor tyrosine kinases and receptor serine/threonine kinases, are major transmembrane signal transducers of many processes such as cell proliferation, differentiation, migration, apoptosis, and defense. In animal systems, receptor tyrosine kinases appear to be predominant in cell signaling. In plants, almost all receptor-like kinases (RLK) identified have serine/threonine specificity, making plants particularly amenable to studying receptor serine/threonine kinase-mediated signaling. The first rice disease resistance gene Xa21, which encodes a RLK, was cloned. The putative intracellular domain of XA21 can autophosphorylate multiple serine and threonine residues and interacts with seven proteins (XBs) in a yeast two-hybrid system. One of the seven binding proteins, XB1, physically interacts with and acts as a substrate of the XA21 kinase. XB1 consists of two domains: an ankyrin domain involved in interacting with XA21 and a RING finger motif belonging to the newly defined R-box family that may play a universal role in ubiquitin-mediated protein degradation. These studies provide an excellent system to study mechanisms underlying initiation, propagation, and termination of RLK-mediated signaling. The specific goals of this project are: 1. Investigate the mode of regulation of the XA21-mediated signaling2. Investigate the role of XBs in the XA21-mediated signaling Achieving these goals will provide insights into RLK-mediated signaling and will advance our understanding of plant disease resistance at the molecular level. Information from these studies would provide new approaches for the engineering of broad-spectrum, durable disease resistance in rice and other crop plants, thereby reducing the dependency of crop production on environmentally unfriendly chemicals.

这个项目的长期目标是了解植物中受体丝氨酸/苏氨酸激酶介导的信号转导。受体激酶包括受体酪氨酸激酶和受体丝氨酸/苏氨酸激酶，是细胞增殖、分化、迁移、凋亡和防御等过程中的主要跨膜信号转导分子。在动物系统中，受体酪氨酸激酶似乎在细胞信号传递中占主导地位。在植物中，几乎所有已发现的受体样激酶(RLK)都具有丝氨酸/苏氨酸特异性，这使得植物特别适合研究受体丝氨酸/苏氨酸激酶介导的信号转导。克隆了第一个水稻抗病基因Xa21，该基因编码一个RLK。在酵母双杂交系统中，Xa21的胞内结构域可以自动磷酸化多个丝氨酸和苏氨酸残基，并与七种蛋白质(XB)相互作用。XB1是七种结合蛋白之一，它与XA21激酶相互作用，并作为其底物。XB1由两个结构域组成：一个是与XA21相互作用的锚蛋白结构域，另一个是属于新定义的R-box家族的环指基序，它可能在泛素介导的蛋白质降解中发挥普遍作用。这些研究为研究RLK介导的信号的启动、传播和终止机制提供了一个很好的系统。本项目的具体目标是：1.研究Xa21介导的信号转导的调控方式。研究xbs在Xa21介导的信号转导中的作用，实现这些目标将有助于我们深入了解RLK介导的信号转导，并将在分子水平上促进我们对植物抗病的理解。来自这些研究的信息将为在水稻和其他农作物中进行广谱、持久的抗病工程提供新的方法，从而减少作物生产对不利于环境的化学品的依赖。