XYZ on a Chip: Integrated Microfluidic Analysis System

XYZ on a Chip：集成微流体分析系统

• 批准号: 0088649
• 负责人: Stephen Quake
• 金额: $ 51万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0088649&HistoricalAwards=false
• 关键词: XYZ; Chip; Integrated; Microfluidic; Analysis

Principal Investigator: Stephen R. Quake, CalTechProposal Number: 0088649Abstract:In this research, chips will be fabricated by monolithic integration of replication molded fluidics with optics and active detectors. These devices will consist of flow channels, including valves and pumps, made from silicone elastomer. In the simplest design, these will be aligned onto micro-optic detector chips with filtered p-n diode detectors and diffractive optic lenses for coupling light into and out of the chip in order to perform fluorescence measurements and manipulation of sub-nanoliters volumes of fluid. The integration of optics with replication molded microfluidics is expected to lead to the construction of very compact but versatile biological testing systems.The main application for these chips will be rapid analysis of cellular information, either of gene expression pattern by RNA analysis or of protein levels through antibody assays. It is also anticipated that this effort will enable the construction of inexpensive and disposable multifunctional bio-sensor chips compact enough to ultimately be implanted into a host. Since valves and pumps are already integrated into the microfluidic system, the resulting chips can concentrate and measure pathogens or toxins as well as deliver drugs in-situ to the host, or perform complex chemical and biological analyses.

摘要：在本研究中，芯片将通过复制模态流体与光学和主动探测器的单片集成来制造。这些装置将由流动通道组成，包括阀门和泵，由硅弹性体制成。在最简单的设计中，这些将对齐到带有滤波p-n二极管探测器和衍射光学透镜的微光学探测器芯片上，用于耦合进出芯片的光，以便进行荧光测量和亚纳升体积流体的操作。光学与复制模塑微流体的集成有望导致非常紧凑但多功能的生物测试系统的建设。这些芯片的主要应用将是细胞信息的快速分析，无论是通过RNA分析基因表达模式还是通过抗体分析蛋白质水平。人们还预计，这一努力将使廉价的一次性多功能生物传感器芯片的构建变得足够紧凑，最终可以植入宿主体内。由于阀门和泵已经集成到微流体系统中，由此产生的芯片可以集中和测量病原体或毒素，以及将药物原位输送到宿主，或执行复杂的化学和生物分析。